PXD025753-1

PXD025753 is an original dataset announced via ProteomeXchange.

Title	MDA-MB-231 MS analysis of ISGylome, GDI2 interactome and total proteome changes in different ISGylation models
Description	Analysis of ISG15 conjugation (Isgylation)role as driver of poor prognosis in non HER2+ breast cancer tumours. The project includes an analysis of total proteome and ISGylome characterization of MDA-MB-231 cells, wild-type or knock-out for the ISGyltaion associated genes, ISG15, UBE2L6 and USP18. Additionally interactome analysis of the ISGylation target GDI2 was perfored in the same lines.
HostingRepository	PRIDE
AnnounceDate	2021-11-03
AnnouncementXML	Submission_2021-11-03_05:52:02.252.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alfonso Bolado
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-02 23:34:07	ID requested
⏵ 1	2021-11-03 05:52:03	announced

Bolado-Carrancio A, Lee M, Ewing A, Muir M, Macleod KG, Gallagher WM, Nguyen LK, Carragher NO, Semple CA, Brunton VG, Caswell PT, von Kriegsheim A, ISGylation drives basal breast tumour progression by promoting EGFR recycling and Akt signalling. Oncogene, 40(44):6235-6247(2021) [pubmed]

submitter keyword: ISG15, Breast cancer, GDI2, EGFR recycling

Alexander von Kriegsheim
contact affiliation	Edinburgh Cancer Research Centre Principal investigator and Mass Spectrometry Facility Manager
contact email	Alex.VonKriegsheim@igmm.ed.ac.uk
lab head
Alfonso Bolado
contact affiliation	University of Edinburgh
contact email	Alfonso.Bolado@igmm.ed.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD025753

PRIDE project URI

• PRIDE
  1. PXD025753
     1. Label: PRIDE project
     2. Name: MDA-MB-231 MS analysis of ISGylome, GDI2 interactome and total proteome changes in different ISGylation models