PXD025737-2

PXD025737 is an original dataset announced via ProteomeXchange.

Title	RhoJ controls EMT associated resistance to chemotherapy
Description	The resistance of cancer cell to therapy is responsible of the death of most cancer patients. Epithelial to mesenchymal transitions (EMT) has been associated with resistance to therapy in different cancer cells. However, the mechanisms by which EMT mediates resistance to therapy remain poorly understood. Here, using a mouse model of skin squamous cell carcinoma (SCC) undergoing spontaneous EMT during tumorigenesis, we identified EMT tumor subpopulations displaying different intrinsic resistance mechanisms to a wide range of anti-cancer therapy both in-vivo and in-vitro. We found that RhoJ, a small GTPase, preferentially expressed in EMT cancer cells controls resistance to therapy. Using genome-wide transcriptomic and proteomic profiling combined with functional assays following loss of RhoJ functions, we found that RhoJ regulated EMT associated resistance to chemotherapy by promoting DNA damage response and the formation of new replication forks allowing EMT tumor cells to repair DNA lesions more rapidly and survive under the replicative stress induced by chemotherapy. Altogether, our study uncovers RhoJ as a key regulator of EMT associated resistance to chemotherapy with important implications for the development of new strategies to overcome resistance to therapy in cancer.
HostingRepository	PRIDE
AnnounceDate	2023-04-17
AnnouncementXML	Submission_2023-04-17_05:52:14.630.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	DelphiVan Haver
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-30 03:27:54	ID requested
1	2023-01-13 01:19:03	announced
⏵ 2	2023-04-17 05:52:15	announced	2023-04-17: Updated project metadata.
3	2023-11-14 08:45:04	announced	2023-11-14: Updated project metadata.

Debaugnies M, Rodr, í, guez-Acebes S, Blondeau J, Parent MA, Zocco M, Song Y, de Maertelaer V, Moers V, Latil M, Dubois C, Coulonval K, Impens F, Van Haver D, Dufour S, Uemura A, Sotiropoulou PA, M, é, ndez J, Blanpain C, RHOJ controls EMT-associated resistance to chemotherapy. Nature, 616(7955):168-175(2023) [pubmed]

submitter keyword: Cancer

EMT

Therapy resistance

RHOJ

CédricBlanpain
contact affiliation	Université libre de Buxelles (ULB), Laboratory of Stem Cells and Cancer, 808 route de Lennik, 1070 Brussels, Belgium ; WELBIO, Université Libre de Bruxelles (ULB), 1070 Bruxelles, Belgium
contact email	Cedric.blanpain@ulb.ac.be
lab head
DelphiVan Haver
contact affiliation	VIB Proteomics Core
contact email	delphi.vanhaver@vib-ugent.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/01/PXD025737

PRIDE project URI

• PRIDE
  1. PXD025737
     1. Label: PRIDE project
     2. Name: RhoJ controls EMT associated resistance to chemotherapy