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PXD025737 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRhoJ controls EMT associated resistance to chemotherapy
DescriptionThe resistance of cancer cell to therapy is responsible of the death of most cancer patients. Epithelial to mesenchymal transitions (EMT) has been associated with resistance to therapy in different cancer cells. However, the mechanisms by which EMT mediates resistance to therapy remain poorly understood. Here, using a mouse model of skin squamous cell carcinoma (SCC) undergoing spontaneous EMT during tumorigenesis, we identified EMT tumor subpopulations displaying different intrinsic resistance mechanisms to a wide range of anti-cancer therapy both in-vivo and in-vitro. We found that RhoJ, a small GTPase, preferentially expressed in EMT cancer cells controls resistance to therapy. Using genome-wide transcriptomic and proteomic profiling combined with functional assays following loss of RhoJ functions, we found that RhoJ regulated EMT associated resistance to chemotherapy by promoting DNA damage response and the formation of new replication forks allowing EMT tumor cells to repair DNA lesions more rapidly and survive under the replicative stress induced by chemotherapy. Altogether, our study uncovers RhoJ as a key regulator of EMT associated resistance to chemotherapy with important implications for the development of new strategies to overcome resistance to therapy in cancer.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDelphi Van Haver
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-04-30 03:27:54ID requested
12023-01-13 01:19:03announced
22023-04-17 05:52:15announced2023-04-17: Updated project metadata.
32023-11-14 08:45:04announced2023-11-14: Updated project metadata.
Publication List
Debaugnies M, Rodr, í, guez-Acebes S, Blondeau J, Parent MA, Zocco M, Song Y, de Maertelaer V, Moers V, Latil M, Dubois C, Coulonval K, Impens F, Van Haver D, Dufour S, Uemura A, Sotiropoulou PA, M, é, ndez J, Blanpain C, RHOJ controls EMT-associated resistance to chemotherapy. Nature, 616(7955):168-175(2023) [pubmed]
Keyword List
submitter keyword: Cancer
Therapy resistance
Contact List
Cédric Blanpain
contact affiliationUniversité libre de Buxelles (ULB), Laboratory of Stem Cells and Cancer, 808 route de Lennik, 1070 Brussels, Belgium ; WELBIO, Université Libre de Bruxelles (ULB), 1070 Bruxelles, Belgium
contact emailCedric.blanpain@ulb.ac.be
lab head
Delphi Van Haver
contact affiliationVIB Proteomics Core
contact emaildelphi.vanhaver@vib-ugent.be
dataset submitter
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