PXD025674-1

PXD025674 is an original dataset announced via ProteomeXchange.

Title	Rne-HupB interaction fosters mycobacterial single-cell homeostasis and fitness during drug treatment and infection
Description	RNA turnover is a primary source of gene expression variation, in turn promoting cellular adaptation. Mycobacteria leverage reversible mRNA stabilization to endure hostile conditions. Although ribonuclease E is essential for RNA turnover in several species, its role in mycobacterial single cell physiology and functional phenotypic diversification remains unexplored. Here, by integrating live-single-cell and quantitative-mass-spectrometry approaches, we show that ribonuclease E forms dynamic foci, which are associated with cellular homeostasis and single-cell fate, and we discover a versatile molecular interactome. We prove the interaction between ribonuclease E and the nucleoid-associated protein HupB, which is particularly pronounced during drug treatment and intracellularly, where we also observed marked increase of phenotypic diversity. Disruption of ribonuclease E expression affects HupB levels, impairing Mycobacterium tuberculosis growth homeostasis during treatment, intracellular replication and host spread. Our work lays the foundation for rational drug design against Mycobacterium tuberculosis diversification capacity, undermining its cellular balance and fitness landscape.
HostingRepository	PRIDE
AnnounceDate	2022-03-25
AnnouncementXML	Submission_2022-03-25_01:24:58.265.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thibaut Douché
SpeciesList	scientific name: Mycolicibacterium smegmatis MC2 155; NCBI TaxID: 246196;
ModificationList	L-methionine (S)-sulfoxide; mono N-acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-27 22:18:28	ID requested
⏵ 1	2022-03-25 01:24:58	announced

Dataset with its publication pending

submitter keyword: Mycobacterium smegmatis, AP-MS, shotgun proteomics, label-free, Q Exactive Plus

Giulia Manina
contact affiliation	Microbial Individuality and Infection Group, Institut Pasteur, Paris, France
contact email	giulia.manina@pasteur.fr
lab head
Thibaut Douché
contact affiliation	Institut Pasteur
contact email	thibaut.douche@pasteur.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/03/PXD025674

PRIDE project URI

• PRIDE
  1. PXD025674
     1. Label: PRIDE project
     2. Name: Rne-HupB interaction fosters mycobacterial single-cell homeostasis and fitness during drug treatment and infection