PXD025643-1

PXD025643 is an original dataset announced via ProteomeXchange.

Title	Proteomic characterization of neuromelanin granules and substantia nigra in health and disease
Description	Neuromelanin is a black-brownish pigment, present in so-called neuromelanin granules (NMG) in the cell bodies of dopaminergic neurons in the substantia nigra. These neurons are lost in neurodegenerative diseases like Parkinson’s disease and dementia with Lewy bodies (DLB). Although it is known that lipids, proteins, and environmental toxins accumulate in NMG, the function of NMG in health and disease remains elusive, as well as the synthesis of neuromelanin. We therefore isolated NMG and surrounding SN tissue from control and DLB patients by laser micro-dissection and analyzed the proteomic profile by tandem-mass spectrometry. The NMG core proteome consisted of several proteins specific for stress granules regardless of health status, indicating a potential, yet unknown, association of NMG and stress granules. Potential-disease related proteomic alterations lead us to the hypothesis of an eventual impairment of the lipid metabolism, as associated proteins were lower abundant in SN tissue of DLB patients. Furthermore, α-synuclein was found to be specifically higher abundant in NMG of DLB patients. As α-synuclein is known to form neurotoxic aggregates, the accumulation inside of NMG suggests a neuroprotective role of NMG in synucleinopathies. With our results, we are able to propose alternative hypotheses on NMG origin and function.
HostingRepository	PRIDE
AnnounceDate	2021-06-07
AnnouncementXML	Submission_2021-06-07_05:15:46.886.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Britta Eggers
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-26 07:00:56	ID requested
⏵ 1	2021-06-07 05:15:47	announced
2	2021-06-07 06:10:54	announced	2021-06-07: Updated project metadata.
3	2021-06-07 06:37:04	announced	2021-06-07: Updated project metadata.

Plum S, Eggers B, Helling S, Stepath M, Theiss C, Leite REP, Molina M, Grinberg LT, Riederer P, Gerlach M, May C, Marcus K, Proteomic Characterization of Synaptosomes from Human Substantia Nigra Indicates Altered Mitochondrial Translation in Parkinson's Disease. Cells, 9(12):(2020) [pubmed]

submitter keyword: Neuromelanin granules, substantia nigra, neurodegeneration, dementia with Lewy bodies, proteomics, stress granules

Katrin Marcus
contact affiliation	Medizinisches Proteom-Center, Medical Faculty, Ruhr-University Bochum, Bochum, Germany Medical Proteome Analysis, Center for Proteindiagnostics (PRODI), Ruhr-University Bochum, Bochum, Germany
contact email	katrin.marcus@rub.de
lab head
Britta Eggers
contact affiliation	Ruhr University Bochum
contact email	britta.eggers@rub.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/06/PXD025643

PRIDE project URI

• PRIDE
  1. PXD025643
     1. Label: PRIDE project
     2. Name: Proteomic characterization of neuromelanin granules and substantia nigra in health and disease