PXD025618-1

PXD025618 is an original dataset announced via ProteomeXchange.

Title	Disruption of nucleocytoplasmic trafficking as a cellular senescence driver
Description	Senescent cells exhibit a reduced response to intrinsic and extrinsic stimuli. This reduction could be explained by disrupted nuclear transmission of signals. However, this hypothesis required more evidence to complete as a new modality of cellular senescence. Proteomic analysis of the cytoplasmic and nuclear fractions from young and senescent cells revealed disruption of nucleocytoplasmic trafficking (NCT) as an essential feature of replicative senescence (RS) at the global level. Blocking NCT either chemically or genetically induced RS-like senescence phenotypes, named as nuclear barrier-induced senescence (NBIS). Transcriptomic analysis revealed that NBIS had the most similar gene expression pattern to RS, compared with other stress-induced types of cellular senescence. Core proteomic and transcriptomic shared patterns between RS and NBIS included upregulation of endocytosis-lysosome network and downregulation of NCT in senescent cells, which were also conserved in yeast aging model. These results implicate an aging-dependent coordinated reduction in the transmission of extrinsic signals to the nucleus and in the nucleus-to-cytoplasm supply of proteins/RNAs. We further showed that the aging-associated decrease in Sp1 transcription factor expression was responsible for downregulation of NCT. Our results suggest that NBIS is a modality of cellular senescence that can represent the nature of physiological aging in eukaryotes.
HostingRepository	PRIDE
AnnounceDate	2021-06-18
AnnouncementXML	Submission_2021-06-17_19:43:30.243.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD025618
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ji-Hwan Park
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	carbamoylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-25 22:11:10	ID requested
⏵ 1	2021-06-17 19:43:30	announced

Dataset with its publication pending

submitter keyword: cellular senescence, replicative senescence, nucleocytoplasmic trafficking, nuclear barrier, eukaryotic aging

Daehee Hwang
contact affiliation	Department of Biological Sciences, Seoul National University, Seoul 08826, Republic of Korea
contact email	daehee@snu.ac.kr
lab head
Ji-Hwan Park
contact affiliation	Korea Bioinformation Center (KOBIC), Korea Research Institute of Bioscience and Biotechnology (KRIBB)
contact email	kp706@postech.ac.kr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD025618
     1. Label: PRIDE project
     2. Name: Disruption of nucleocytoplasmic trafficking as a cellular senescence driver