PXD025569 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Unbiased quantitative analysis of the cardiac phosphoproteome in response to acute β-adrenergic receptor stimulation in vivo |
Description | We utilised Super-SILAC mouse technology to perform an unbiased, quantitative analysis of the cardiac phosphoproteome in mice acutely treated in vivo with either saline or isoprenaline (ISO), a non-selective β-AR agonist. Using a customized mass spectrometry-based workflow (Figure 1), our study identified hundreds of phosphosites that are significantly regulated by β-AR stimulation, including both novel phosphoproteins and phosphosites that have not been previously identified as downstream effectors of β-AR stimulation and established downstream components of cAMP/PKA and CaMKII signaling pathways. Interestingly, our study revealed both up- and down-phosphorylation in comparable numbers, indicative of an intricate signaling network activated in response to β-AR stimulation. |
HostingRepository | PRIDE |
AnnounceDate | 2021-12-30 |
AnnouncementXML | Submission_2021-12-30_14:51:45.967.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Christof Lenz |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-04-22 23:09:14 | ID requested | |
⏵ 1 | 2021-12-30 14:51:46 | announced | |
Publication List
G, ü, ran A, Ji Y, Fang P, Pan KT, Urlaub H, Avkiran M, Lenz C, -Adrenergic Receptor Stimulation In Vivo. Int J Mol Sci, 22(22):(2021) [pubmed] |
Keyword List
submitter keyword: phosphoproteome |
mass spectrometry |
β-adrenergic receptor |
SILAC |
Contact List
Christof Lenz |
contact affiliation | Institute of Clinical Chemistry, University Medical Center Göttingen |
contact email | christof.lenz@mpibpc.mpg.de |
lab head | |
Christof Lenz |
contact affiliation | Max Planck Institute for Biophysical Chemistry |
contact email | christof.lenz@mpibpc.mpg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD025569
- Label: PRIDE project
- Name: Unbiased quantitative analysis of the cardiac phosphoproteome in response to acute β-adrenergic receptor stimulation in vivo