PXD025537-1

PXD025537 is an original dataset announced via ProteomeXchange.

Title	On-tissue spatially-resolved glycoproteomics guided by N-glycan imaging reveal global dysregulation of canine glioma glycoproteomic landscape
Description	Here we present an approach to identify N-linked glycoproteins and deduce their spatial localization using a combination of MALDI N-glycan MSI and spatially-resolved glycoproteomics. We subjected glioma biopsies to on-tissue PNGaseF digestion and MALDI-MSI and found that the glycan HexNAc4-Hex5-NeuAc2 was predominantly expressed in necrotic regions of high-grade canine gliomas. To determine the underlying sialo-glycoprotein, various regions in adjacent tissue sections were subjected to microdigestion and manual glycoproteomic analysis. Results identified haptoglobin as the protein associated with HexNAc4-Hex5-NeuAc2, making our study the first report that directly links glycan imaging with intact glycopeptide identification. In total, our spatially-resolved glycoproteomics technique identified over 400 N-, O-, and S- glycopeptides from over 30 proteins, demonstrating the diverse array of glycosylation present on the tissue slides and the sensitivity of our technique. Ultimately, this proof-of-principle work demonstrates that spatially-resolved glycoproteomics greatly complement MALDI-MSI in understanding dysregulated glycosylation.
HostingRepository	PRIDE
AnnounceDate	2022-05-04
AnnouncementXML	Submission_2022-05-04_04:30:56.949.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stacy Malaker
SpeciesList	scientific name: Canis lupus familiaris; NCBI TaxID: 9615;
ModificationList	complex glycosylation
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-21 09:17:30	ID requested
⏵ 1	2022-05-04 04:30:57	announced
2	2023-11-14 08:52:41	announced	2023-11-14: Updated project metadata.

10.1016/J.CHEMBIOL.2021.05.007;

submitter keyword: glycan imaging, MALDI-MSI, glycoproteomics, haptoglobin, glioblastoma, sialic acid, hypersialylation, spatially resolved glycoproteomicsglycan imaging, MALDI-MSI, glycoproteomics, haptoglobin, glioblastoma, sialic acid, hypersialylation, spatially resolved glycoproteomicsglycan imaging, MALDI-MSI, glycoproteomics, haptoglobin, glioblastoma, sialic acid, hypersialylation, spatially resolved glycoproteomics

Stacy Malaker
contact affiliation	Yale University
contact email	stacy.malaker@yale.edu
lab head
Stacy Malaker
contact affiliation	Yale University
contact email	stacy.malaker@yale.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD025537

PRIDE project URI

• PRIDE
  1. PXD025537
     1. Label: PRIDE project
     2. Name: On-tissue spatially-resolved glycoproteomics guided by N-glycan imaging reveal global dysregulation of canine glioma glycoproteomic landscape