PXD025515 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | TMT-based quantitative proteomics of exosomes from porcine colostrum and milk |
Description | Colostrum and milk have high nutritional value and provide a complete diet for neonates, along with bioactive substances which modulate various functions such as immune defense. Exosomes are membranous vesicles of endocytic origin, recently been considered as major players in cell-cell communication. The mechanisms by which milk components can prime the infant’s active immunity are not entirely clear, and exosomes are suggested to be essential for the infant’s physiological development. We assessed the exosomal proteome profile from milk samples obtained from 10 healthy sows, at day 0 (colostrum), day 7, and 14 post-partum. Exosomes were isolated by ultracentrifugation coupled with size exclusion chromatography, and were characterized by nanoparticle tracking analysis, transmission electron microscopy and Western blotting for exosome markers. Isolated exosomes were in-gel digested and after TMT-labelling of the peptides, they were subjected to LC-MS/MS. Quantitative proteomics analysis revealed different proteome profiles for colostrum exosomes and milk exosomes, and functional analysis highlighted pathways related to immune response, cellular development, and regulation of cellular processes. This study endorses the importance of exosomes as active biocomponents of milk and provides knowledge for future studies exploring their role in regulation of immunity and growth of the newborn. |
HostingRepository | PRIDE |
AnnounceDate | 2022-02-08 |
AnnouncementXML | Submission_2022-02-08_04:52:40.263.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD025515 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Marc Sylvester |
SpeciesList | scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-04-21 02:42:34 | ID requested | |
⏵ 1 | 2022-02-08 04:52:41 | announced | |
Publication List
Ferreira RF, Blees T, Shakeri F, Buness A, Sylvester M, Savoini G, Agazzi A, Mrljak V, Sauerwein H, Comparative proteome profiling in exosomes derived from porcine colostrum versus mature milk reveals distinct functional proteomes. J Proteomics, 249():104338(2021) [pubmed] |
Keyword List
submitter keyword: orcine, exosomes, extracellular vesicles, LC-MS/MS, TMT labelling, milk, colostrum, pig |
Contact List
Helga Sauerwein |
contact affiliation | University of Bonn, Germany |
contact email | sauerwein@uni-bonn.de |
lab head | |
Marc Sylvester |
contact affiliation | Institute of Biochemistry and Molecular Biology, Medical Faculty, University of Bonn |
contact email | msylvest@uni-bonn.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD025515
- Label: PRIDE project
- Name: TMT-based quantitative proteomics of exosomes from porcine colostrum and milk