PXD025481-1

PXD025481 is an original dataset announced via ProteomeXchange.

Title	Single cell chemical proteomics (SCCP) interrogates the timing and heterogeneity of cancer cell commitment to death
Description	Chemical proteomics studies the effects of drugs upon cellular proteome. Due to the complexity and diversity of tumors, the response of cancer cells to drugs is also heterogeneous, and thus proteome analysis at single cell level is needed. So far, single cell proteomics techniques have not been quantitative enough to tackle the drug effects on the target proteins. Here, we developed the first single cell chemical proteomics (SCCP) technique and studied with it the time-resolved response to anticancer drugs methotrexate, camptothecin and tomudex of individual adenocarcinoma A549 cells. For each drug SCCP identified and quantified 1,000-2,000 proteins across >150 single cells in a time course of treatment up to 48 h, revealing the early emergence of cellular subpopulations committed and uncommitted to death. SCCP represents a novel approach to exploring the heterogeneous response to drugs of cancer cells.
HostingRepository	PRIDE
AnnounceDate	2022-07-01
AnnouncementXML	Submission_2022-07-01_11:59:39.052.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	JIMMY RODRIGUEZ
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-19 22:04:21	ID requested
⏵ 1	2022-07-01 11:59:39	announced
2	2023-11-14 08:52:57	announced	2023-11-14: Updated project metadata.

10.1021/ACS.ANALCHEM.2C00413;

submitter keyword: TMT labeling

Drug sensitivity

Resistance to drugs

Cell death

Methotrexate

Camptothecin

Tomudex

Roman Zubarev
contact affiliation	Division of Chemistry I, Department of Medical Biochemistry & Biophysics, Karolinska Institutet, Biomedicum A9, Solnavägen 9, SE-171 77 Stockholm, Sweden
contact email	roman.zubarev@ki.se
lab head
JIMMY RODRIGUEZ
contact affiliation	Division of Chemistry I, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
contact email	esneider007@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/07/PXD025481

PRIDE project URI

• PRIDE
  1. PXD025481
     1. Label: PRIDE project
     2. Name: Single cell chemical proteomics (SCCP) interrogates the timing and heterogeneity of cancer cell commitment to death