PXD025445-1

PXD025445 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Oncogene Regulated Release of Extracellular Vesicles
Description	Oncogenes can alter metabolism by changing the balance between anabolic and catabolic processes. However, how oncogenes regulate tumor cell biomass remains poorly understood. Using isogenic MCF10A cells transformed with nine different oncogenes, we show that specific oncogenes reduce the biomass of cancer cells by promoting EV release. While MYC and AURKB elicited the highest number of EVs, each oncogene selectively altered the protein composition of released EVs. Likewise, oncogenes alter secreted miRNAs. MYC overexpressing cells require ceramide, while AURKB require ESCRT to release high levels of EVs. We identify an inverse relationship between MYC upregulation and activation of the RAS/MEK/ERK signaling pathway for regulating EV release in some tumor cells. Finally, lysosome genes and activity are downregulated in the context of MYC and AURKB, suggesting that cellular contents instead of being degraded, were released via EVs. Thus, oncogene mediated biomass regulation via differential EV release is a new metabolic phenotype.
HostingRepository	MassIVE
AnnounceDate	2021-04-16
AnnouncementXML	Submission_2021-04-16_15:48:57.066.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Brett Phinney
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	Carbamidomethyl; Oxidation
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-16 12:48:21	ID requested
⏵ 1	2021-04-16 15:48:57	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: oncogenes, extracellular vesicles (EVs), ceramide, ESCRT, MYC, HRAS, AURKB, biomass, lysosome

submitter keyword: oncogenes, extracellular vesicles (EVs), ceramide, ESCRT, MYC, HRAS, AURKB, biomass, lysosome

Contact List

Andrei Goga
contact affiliation	Department of Cell & Tissue Biology, University of California, San Francisco
contact email	Andrei.Goga@ucsf.edu
lab head
Brett Phinney
contact affiliation	UC Davis
contact email	brettsp@ucdavis.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000087219/