PXD025405-1

PXD025405 is an original dataset announced via ProteomeXchange.

Title	Resource sharing by outer membrane vesicles from a citrus pathogen
Description	The causative agent of citrus canker disease, Xanthomonas citri pv. citri, was found to produce copious amounts of outer membrane vesicles (OMVs), frequently forming long membranous tubes under different culture conditions. Lipidomic analysis revealed significant differences in lipid composition between purified vesicles in relation to whole cells. The results suggest an enrichment in saturated cardiolipins and a decrease in unsaturated lipids in the OMV samples, possibly granting them a more rigid structure while allowing their high degree of curvature caused by their small diameters. The vesicles’ proteome was found to be significantly enriched in TonB-dependent receptors related to the acquisition of different nutrients, including siderophores. These iron-binding molecules were evidenced to be present in purified X. citri OMVs, along with essential metals including iron itself, zinc, and manganese quantified by elemental analysis. The availability of vesicle-associated nutrients to be incorporated by cells was demonstrated by the use of OMVs as the sole carbon source for bacterial growth. At last, the vesicles also presented esterase and protease activities, which have been associated with virulence in phytopathogens. These evidences point that X. citri cells can use OMVs to share resources within microbial communities, which has potential implications for microbial interactions and plant colonization, affecting their survival and persistence on the host and in the environment.
HostingRepository	PRIDE
AnnounceDate	2025-07-28
AnnouncementXML	Submission_2025-07-27_16:04:49.745.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Giuseppe Palmisano
SpeciesList	scientific name: Xanthomonas citri pv. citri str. 306; NCBI TaxID: 190486;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-14 21:21:30	ID requested
⏵ 1	2025-07-27 16:04:50	announced

Araujo GG, Conforte MM, da Purifica, ç, ã, o AD, Todeschini I, Llontop EE, Angeli CB, Inague A, Yoshinaga MY, de Souza RF, Papai R, Luz MS, Miyamoto S, Palmisano G, Farah CS, Guzzo CR, Functional Cargo in Membrane Vesicles From a Citrus Pathogen. Environ Microbiol Rep, 17(4):e70101(2025) [pubmed]

10.1111/1758-2229.70101;

submitter keyword: proteomics,outer membrane vesicles, TonB, receptors, xanthomonas

Cristiane Guzzo Carvalho
contact affiliation	Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo
contact email	crisguzzo@usp.br
lab head
Giuseppe Palmisano
contact affiliation	University of Sao Paulo
contact email	palmisano.gp@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD025405

PRIDE project URI

• PRIDE
  1. PXD025405
     1. Label: PRIDE project
     2. Name: Resource sharing by outer membrane vesicles from a citrus pathogen