PXD025383-1

PXD025383 is an original dataset announced via ProteomeXchange.

Title	The chimeric GaaR::XlnR transcription factor induces pectinolytic activities in the presence of D-xylose in Aspergillus niger
Description	Aspergillus niger is a filamentous fungus well known for its ability to produce a wide variety of pectinolytic enzymes, which have many applications in the industry. The transcriptional activator GaaR is induced by 2-keto-3-deoxy-L-galactonate, a compound derived from D-galacturonic acid, and plays a major role in the regulation of pectinolytic genes. The requirement for inducer molecules can be a limiting factor for the production of enzymes. Therefore, the generation of chimeric transcription factors able to activate the expression of pectinolytic genes by using underutilized agricultural residues would be highly valuable for industrial applications. In this study, we used the CRISPR/Cas9 system to generate three chimeric GaaR::XlnR transcription factors by swapping the N-terminal region of the xylanolytic regulator XlnR to that of the GaaR in A. niger. As a test case, we constructed a PpgaX:hph reporter strain to evaluate the alteration of transcription factor specificity in the chimeric mutants. Additionally, we generated a constitutively active GaaR::XlnR V756F version of the most efficient chimeric transcription factor to better assess its activity. Our results showed that the chimeric GaaR::XlnR transcription factor was no longer induced in the presence of D-galacturonic acid, but in the presence of D-xylose instead. Moreover, proteomics analysis and saccharification assays confirmed the production of enzymes involved in the release of L-arabinose from pectin, while the constitutive version of this chimeric transcription factor showed consistently improved D-galacturonic acid release from pectin in a gaaR deletion background.
HostingRepository	PRIDE
AnnounceDate	2021-11-18
AnnouncementXML	Submission_2021-11-18_06:50:37.590.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD025383
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Marcos Di Falco
SpeciesList	scientific name: Aspergillus niger NRRL3; NCBI TaxID: 1451139;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-14 01:56:27	ID requested
⏵ 1	2021-11-18 06:50:37	announced

Dataset with its publication pending

submitter keyword: Aspergillus niger, chimeric transcription factor, CRISPR/Cas9, pectinases, D-xylose

Ronald P. deVries
contact affiliation	Westerdijik Fungal Biodiversity Institute Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
contact email	r.devries@wiknaw.nl
lab head
Marcos Di Falco
contact affiliation	Concordia University
contact email	marcos.difalco@concordia.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD025383
     1. Label: PRIDE project
     2. Name: The chimeric GaaR::XlnR transcription factor induces pectinolytic activities in the presence of D-xylose in Aspergillus niger