PXD025326-1

PXD025326 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	An Acquired Acyltransferase Promotes Klebsiella pneumoniae ST258 Respiratory Infection
Description	Klebsiella pneumoniae ST258 are human pathogens associated with poor outcomes in patients worldwide. We identified a member of the acyltransferase superfamily 3 (atf3), enriched within the ST258 clade, that provides a major competitive advantage for the proliferation of this group of organisms in vivo. Comparison of a wild type ST258 strain (KP35) and a atf3 isogenic mutant generated by Crispr-Cas9 targeting, revealed increased NADH:quinone oxidoreductase transcription and ATP generation, fueled by increased glycolysis. Acquisition of atf3 induced changes in the bacterial acetylome, promoting lysine acetylation of multiple gene products involved in central metabolism, specifically Zwf (glucose-6 phosphate dehydrogenase). The atf3-mediated metabolic boost led to greater consumption of glucose in the host airway and increased bacterial burden in the lung, independent of cytokine levels and immune cell recruitment. Acquisition of a promiscuous acyltransferase enhances K. pneumoniae ST258 fitness and promotes its emergence as a major health care associated pathogen.
HostingRepository	MassIVE
AnnounceDate	2023-05-26
AnnouncementXML	Submission_2023-05-26_08:08:14.634.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rajesh
SpeciesList	scientific name: Klebsiella pneumoniae;
ModificationList	acetylated residue
Instrument	Orbitrap Fusion

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-12 13:17:00	ID requested
⏵ 1	2023-05-26 08:08:14	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Klebsiella pneumoniae

submitter keyword: Klebsiella pneumoniae

Contact List

Danielle Ahn
contact affiliation	Columbia University - Department of Pediatrics
contact email	dsa2120@cumc.columbia.edu
lab head
Rajesh
contact affiliation	Columbia University
contact email	rs3869@cumc.columbia.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000087181/