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PXD025319-1

PXD025319 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRedox regulation of PTPN22 affects the severity of T cell dependent autoimmune inflammation
DescriptionPTPN22, a modifier of T cell receptor signaling, and reactive oxygen species are major players in the regulation of chronic autoimmune diseases. As with all protein tyrosine phosphatases the activity of PTPN22 is redox regulated, but if or how such regulation can modulate inflammatory pathways in vivo is not known. To determine this, we created a mouse with a cysteine-to-serine mutation at position 129 in PTPN22 (C129S), a residue proposed to alter the redox regulatory properties of PTPN22 by forming a disulfide with the catalytic C227 residue. The C129S mutant mouse showed a stronger T cell-dependent inflammatory response due to enhanced TCR signaling and activation of T cells. Activity assays with purified proteins suggest that the functional results can be explained by an increased sensitivity to oxidation of the C129S mutated PTPN22 protein. We also observed that the disulfide of native PTPN22 can be directly reduced by the thioredoxin system, while the C129S mutant lacking this disulfide was less amenable to reductive reactivation. In conclusion, we show in the mouse that the non-catalytic C129 residue of PTPN22 modulates its redox regulatory properties, and that oxidation-prone PTPN22 leads to increased severity in the development of T cell-dependent autoimmunity.
HostingRepositoryPRIDE
AnnounceDate2022-03-23
AnnouncementXMLSubmission_2022-03-23_07:40:10.573.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAmir Ata Saei
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-04-12 07:59:25ID requested
12022-03-23 07:40:10announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Autoimmunity, PTPN22, reactive oxygen species, lymphocyte signaling
Contact List
Prof. Roman. A. Zubarev
contact affiliationDepartment of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
contact emailRoman.zubarev@ki.se
lab head
Amir Ata Saei
contact affiliationDepartment of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
contact emailamirata.saei.dibavar@ki.se
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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