PXD025305-1

PXD025305 is an original dataset announced via ProteomeXchange.

Title	HSPA1A inhibition in early- and late-passage primary human mesemchymal stem cells subjected to 2 hour heat shock
Description	Cells respond to stress by synthesising chaperone proteins that correct protein misfolding to maintain function. However, protein homeostasis is lost in ageing, leading to aggregates characteristic of protein-folding diseases. Whilst much is known about how these diseases progress, discovering what causes protein-folding to deteriorate could be key to their prevention. Here, we examined primary human mesenchymal stem cells (hMSCs), cultured to a point of replicative senescence and subjected to heatshock, as an in vitro model of the ageing stress response. We found through proteomic analysis that the maintenance of homeostasis deteriorated in senescent cells, coincident with lowered levels of a functional module of chaperone proteins associated with heat shock protein 70 kDa (HSPA1A). Further analysis of the temporal dynamics of the proteomic and transcriptomic stress response revealed a lack of translational capacity to be a limiting factor in the capacity of senescent cells to mitigate proteotoxic stress.
HostingRepository	PRIDE
AnnounceDate	2021-06-15
AnnouncementXML	Submission_2021-06-15_04:59:09.803.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jack Llewellyn
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-09 06:16:33	ID requested
⏵ 1	2021-06-15 04:59:10	announced

Dataset with its publication pending

submitter keyword: Human senescent heat shock MSC mesenchymal stem cell HSP70 inhibition HSPA1A

Joe Swift
contact affiliation	The University of Manchester
contact email	joe.swift@manchester.ac.uk
lab head
Jack Llewellyn
contact affiliation	The University of Manchester
contact email	jdmllewellyn@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD025305
     1. Label: PRIDE project
     2. Name: HSPA1A inhibition in early- and late-passage primary human mesemchymal stem cells subjected to 2 hour heat shock