PXD025300 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Revealing the dynamic allosteric changes required for formation of the cysteine synthase complex by hydrogen-deuterium exchange mass spectrometry |
Description | CysE and CysK, the last two enzymes of the cysteine biosynthetic pathway, engage in a bienzyme complex, cysteine synthase (CS), with yet incompletely characterized three-dimensional structure and regulatory function. Being absent in mammals, the two enzymes and their complex are attractive targets for antibacterial drugs. We have used hydrogen/deuterium exchange mass spectrometry to unveil how complex formation affects the conformational dynamics of CysK and CysE. Our results support a model where CysE is present in solution as a dimer of trimers, and each trimer can bind one CysK homodimer. When CysK binds to one CysE monomer, intra-trimer allosteric communication ensures conformational and dynamic symmetry within the trimer. Furthermore, a longer-range allosteric signal propagates through CysE to induce stabilization of the interface between the two CysE trimers, preparing the second trimer for binding the second CysK with a non-random orientation. These results provide new molecular insights into the allosteric formation of the CS complex and could help guide antibacterial drug design. |
HostingRepository | PRIDE |
AnnounceDate | 2021-06-07 |
AnnouncementXML | Submission_2021-06-07_02:48:33.600.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Brenda Rosa |
SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; |
ModificationList | No PTMs are included in the dataset |
Instrument | Synapt MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-04-09 03:54:51 | ID requested | |
⏵ 1 | 2021-06-07 02:48:34 | announced | |
Publication List
Rosa B, Dickinson ER, Marchetti M, Campanini B, Pioselli B, Bettati S, Rand KD, Revealing the Dynamic Allosteric Changes Required for Formation of the Cysteine Synthase Complex by Hydrogen-Deuterium Exchange MS. Mol Cell Proteomics, 20():100098(2021) [pubmed] |
Keyword List
submitter keyword: Cysteine biosynthesis, bienzyme complex, protein-protein interactions, allosteric regulation, cysteine synthase, E. coli, Synapt G2-Si, Hydrogen deuterium exchange (hdx-ms) |
Contact List
Kasper Dyrberg Rand |
contact affiliation | Protein Analysis Group, Department of Pharmacy, University of Copenhagen, 2100 Copenhagen O, Denmark |
contact email | kasper.rand@sund.ku.dk |
lab head | |
Brenda Rosa |
contact affiliation | University of Parma |
contact email | brenda.rosa@studenti.unipr.it |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD025300
- Label: PRIDE project
- Name: Revealing the dynamic allosteric changes required for formation of the cysteine synthase complex by hydrogen-deuterium exchange mass spectrometry