PXD025275-1

PXD025275 is an original dataset announced via ProteomeXchange.

Title	A dual RNA regulator balancing carbon uptake and 1 central metabolism in Vibrio cholerae
Description	Bacterial small RNAs (sRNAs) are well-known to modulate gene expression by base-pairing with trans-coded transcripts and are typically considered to be non-coding. However, several sRNAs have been reported to also contain an open reading frame and thus are considered dual regulators. In this study, we discovered a dual regulator from Vibrio cholerae, called VcdRP, harboring a 29 amino acid protein (VcdP), as well as a base-pairing sequence. Using a forward genetic screen, we identified VcdRP as a repressor of cholera toxin production and we link this phenotype to inhibition of carbon transport by the base-pairing segment of the regulator. By contrast, we demonstrate that VcdP acts downstream of carbon transport by binding to citrate synthase (GltA), the first enzyme of the citric cycle. Interaction of VcdP with GltA results in increased enzyme activity and together VcdR and VcdP reroute carbon metabolism. We further show that transcription of vcdRP is repressed by CRP allowing us to provide a model in which VcdRP employs two different molecular mechanisms to synchronize sugar uptake and metabolism in V. cholerae.
HostingRepository	PRIDE
AnnounceDate	2021-07-22
AnnouncementXML	Submission_2021-07-22_05:34:01.554.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andreas Tholey
SpeciesList	scientific name: Vibrio cholerae O1 biovar El Tor str. N16961; NCBI TaxID: 243277;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-08 09:16:40	ID requested
⏵ 1	2021-07-22 05:34:01	announced

Dataset with its publication pending

submitter keyword: Vibrio cholerae, dual function RNA regulator, small protein

Prof. Dr.Andreas Tholey
contact affiliation	Systematische Proteomics & Bioanalytik - Institut für Experimentelle Medizin Christian-Albrechts-Universität zu Kiel Niemannsweg 11 24105 Kiel, Germany
contact email	a.tholey@iem.uni-kiel.de
lab head
Andreas Tholey
contact affiliation	Systematic Proteome Research & Bioanalytics, University of Kiel
contact email	a.tholey@iem.uni-kiel.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD025275
     1. Label: PRIDE project
     2. Name: A dual RNA regulator balancing carbon uptake and 1 central metabolism in Vibrio cholerae