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PXD025273-1

PXD025273 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTransplant Model Ischemic Conditioning Causes Tissue Leakage and Suppressed Inflammation in a Porcine Kidney
DescriptionBackground: Remote Ischemic Conditioning (RIC) has been proposed as a therapeutic intervention to circumvent the Ischemia/reperfusion injury (IRI) that is inherent to organ transplantation. Using a porcine kidney transplant model, we aimed to decipher the subclinical molecular effects of a RIC regime, compared to non-RIC controls. Methods: Kidney pairs (n = 8+8) were extracted from brain dead donor pigs and transplanted in juvenile recipient pigs following a period of cold ischemia. One kidney in each pair was subjected to RIC prior to reperfusion, while the other served as non-RIC control. We designed an advanced integrative -Omics strategy combining transcriptomics, proteomics, and phosphoproteomics to deduce molecular signatures in kidney tissue that could be attributed to RIC. Results: In kidney grafts taken out 10 h after transplantation we detected minimal molecular perturbations following RIC compared to non-RIC at the transcriptome level, but more pronounced effects at the proteome level. In particular, we noted that RIC resulted in response suppression of tissue inflammatory profiles. Furthermore, an accumulation of muscle extracellular matrix assembly proteins in RIC tissues was detected at the protein level, which may result in response to muscle tissue damage and/or fibrosis. Conclusions: Our data identifies subtle molecular phenotypes in porcine kidneys subjected to RIC which could aid further optimisation of remote ischaemia protocols in renal transplantation.
HostingRepositoryPRIDE
AnnounceDate2022-05-25
AnnouncementXMLSubmission_2022-05-25_07:09:45.722.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDarragh O'Brien
SpeciesList scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; phosphorylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF; Orbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-04-08 08:03:29ID requested
12022-05-25 07:09:49announced
Publication List
O'Brien DP, Thorne AM, Huang H, Pappalardo E, Yao X, Thyrrestrup PS, Ravlo K, Secher N, Norregaard R, Ploeg RJ, Jespersen B, Kessler BM, Integrative omics reveals subtle molecular perturbations following ischemic conditioning in a porcine kidney transplant model. Clin Proteomics, 19(1):6(2022) [pubmed]
Keyword List
submitter keyword: Remote Ischaemic Conditioning, Kidney Transplantation, Proteomics, Transcriptomics, Integrative Omics, Inflammation
Contact List
Benedikt Kessler
contact affiliationTarget Discovery Institute, Centre for Medicines Discovery, Nuffield Department of Medicine, University of Oxford, UK
contact emailbenedikt.kessler@ndm.ox.ac.uk
lab head
Darragh O'Brien
contact affiliationTarget Discovery Institute, University of Oxford
contact emaildarragh.obrien@ndm.ox.ac.uk
dataset submitter
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