PXD025255 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Automated method for enrichment of newly synthesized proteins based on AHA labeling |
Description | In this study, a novel high throughput method was successfully developed for the automated enrichment of newly synthesized proteins (NSPs) from complex environments, like cell culture media supplemented with fetal bovine serum (FBS). The proposed automated method allows for the robust and effective simultaneous enrichment of dozen of samples in a period of 3 hours. The enrichment method was comprehensively evaluated and validated, and was applied to the mass spectrometry (MS) analysis of the FBS-rich secretome of melanoma A375 cells treated with the immune cytokines interferon alpha (IFN-α) and gamma (IFN-γ). |
HostingRepository | PRIDE |
AnnounceDate | 2023-05-14 |
AnnouncementXML | Submission_2023-05-14_14:20:06.596.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | DavidVargas Diaz |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | 6x(13)C labeled residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-04-08 02:24:28 | ID requested | |
1 | 2022-02-17 00:43:28 | announced | |
⏵ 2 | 2023-05-14 14:20:07 | announced | 2023-05-14: Updated project metadata. |
Publication List
Vargas-Diaz D, Altelaar M, Automated High-Throughput Method for the Fast, Robust, and Reproducible Enrichment of Newly Synthesized Proteins. J Proteome Res, 21(1):189-199(2022) [pubmed] |
Keyword List
ProteomeXchange project tag: EPIC-XS |
submitter keyword: High throughput, newly synthesized proteins, AHA, LC-MS, interferon, melanoma, enrichment, Proteomics, Click chemistry |
Contact List
MaartenAltelaar |
contact affiliation | Professor, Department of Pharmaceutical Sciences, Netherlaands Proteomics Centre, Utrecht University, The Netherlands |
contact email | m.altelaar@uu.nl |
lab head | |
DavidVargas Diaz |
contact affiliation | Utrecht University |
contact email | d.vargasdiaz@uu.nl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD025255 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD025255
- Label: PRIDE project
- Name: Automated method for enrichment of newly synthesized proteins based on AHA labeling