PXD025250 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Stable and temporal interactomes of CBL and CBLB |
| Description | CBL family proteins have been widely reported as negative regulators of various signal pathways, which determines the amplitude and duration of signaling response through mediating the ubiquitination and degradation of activated receptors. CBL and CBLB share high degrees of structural similarities, and they are more than just ubiquitin-protein ligases and contain rich interactive domains and motifs that nucleate the assembly of protein complex taking various cellular functions. However, proteome-wide interaction network mediated by CBL proteins was rarely reported. This study is aimed to explore CBL and CBLB-mediated interactomes, hoping to uncover their regulatory roles in EGFR signaling and provide new insight into the molecular events driving tumorigenesis. With the construction of stable HeLa cell lines inducibly expressing FLAG-tagged CBL or CBLB, we detected both stable interactomes and EGF stimulation-induced temporal interactomes of CBL and CBLB through AP-MS approach. Time-resolved profiling and functional annotations of the temporal interactomes sheds light on the dynamic assemble of signal proteins over the signal cascade. Moreover, comparison between interactomes of CBL and CBLB indicates the redundant but also complementary functions of them. This work offer a useful resource for CBL and CBLB interactome, highlighting their prominent and diverse roles in the EGFR signalsome. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-07-14 |
| AnnouncementXML | Submission_2026-07-14_01:33:23.883.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Jie Liu |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | monohydroxylated residue; deamidated residue |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2021-04-07 23:19:28 | ID requested | |
| ⏵ 1 | 2026-07-14 01:33:24 | announced | |
Publication List
| Liu J, Yang L, He A, Ke M, Fu C, Gao W, Xu R, Tian R, Stable and EGF-Induced Temporal Interactome Profiling of CBL and CBLB Highlights Their Signaling Complex Diversity. J Proteome Res, 20(7):3709-3719(2021) [pubmed] |
| 10.1021/acs.jproteome.1c00284; |
Keyword List
| submitter keyword: CBL, CBLB, signal transduction, dynamic protein complexes,AP-MS |
Contact List
| Ruijun Tian |
|---|
| contact affiliation | Department of Chemistry, College of Science,Southern University of Science and Technology,Shenzhen518055, China |
| contact email | tianrj@sustech.edu.cn |
| lab head | |
| Jie Liu |
|---|
| contact affiliation | Shenzhen Blood Center |
| contact email | liujiebnu2009@163.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD025250 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD025250
- Label: PRIDE project
- Name: Stable and temporal interactomes of CBL and CBLB
