PXD025243-1

PXD025243 is an original dataset announced via ProteomeXchange.

Title	RNA Polymerase II interactome in presence or absence of SPT6 in U2OS cells
Description	SPT6 is a histone chaperone that tightly binds RNA polymerase II (POL2) during transcription elongation. However, its primary role in POL2 transcription is uncertain. We used targeted protein degradation to rapidly deplete SPT6 in human cells and analyzed defects in POL2 behavior by a multi-omics approach and mathematical modeling. Our data indicate that SPT6 is a crucial factor for POL2 processivity and is therefore required for the productive transcription of protein-coding genes. Unexpectedly, SPT6 also has a vital role in POL2 termination, as acute depletion induced readthrough transcription for thousands of genes. Long-term depletion of SPT6 induced cryptic intragenic transcription, as observed earlier in yeast. However, this phenotype was not observed upon acute SPT6 depletion and therefore can be attributed to accumulated epigenetic perturbations in the absence of SPT6. In conclusion, targeted protein degradation of SPT6 allowed the temporal discrimination of its function as an epigenetic safeguard and POL2 elongation factor.
HostingRepository	PRIDE
AnnounceDate	2021-06-28
AnnouncementXML	Submission_2021-06-27_22:31:42.651.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ashwin Narain
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-07 05:39:20	ID requested
⏵ 1	2021-06-27 22:31:43	announced

Dataset with its publication pending

submitter keyword: SPT6, SUPT6H, RNA Polymerase II, Auxin, AID, U2OS

Prof. Elmar Wolf
contact affiliation	Lehrstuhl für Biochemie und Molekularbiologie Biozentrum Am Hubland 97074 Würzburg
contact email	elmar.wolf@biozentrum.uni-wuerzburg.de
lab head
Ashwin Narain
contact affiliation	Julius-Maximilians-Universität Würzburg
contact email	ashwin.narain@uni-wuerzburg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD025243
     1. Label: PRIDE project
     2. Name: RNA Polymerase II interactome in presence or absence of SPT6 in U2OS cells