PXD025241-1

PXD025241 is an original dataset announced via ProteomeXchange.

Title	Tandem mass tag-based proteomic analysis in saliva of horses with acute abdominal disease
Description	The objective of the study was to analyze the changes in proteomics that occur in saliva in acute abdominal disease (AAD) by proteomics in order to identify new proteins that could allow gaining knowledge about the physiopathological changes that can occur in this fluid and identify possible new biomarkers of acute abdominal disease. Samples from two groups of horses were analyzed by TMT proteomic analysis: - Diseased horses (n=7; 4 stallions and 3 mares; mean age = 8 years (range 1-18); Andalusians (n=5) and crossbred (n=2)). Presence of AAD was diagnosed based on history, physical examination (abdominal auscultation, rectal examination and nasogastric intubation) and additional diagnostic tests incl. complete blood count (CBC), serum biochemistry profile, abdominal ultrasound and or abdominocentesis. Final diagnoses were impaction of ascending colon (n=3), right dorsal colon displacement (n=2) and spasmodic colon (n=2). - Healthy horses (n=6, 3 geldings, 2 stallions and 1 mare; mean age = 12 years (range 4-15); Andalusians (n=4) and crossbred (n=2)). Horses were found healthy based on history, clinical examination, CBC and serum biochemistry profile. These procedures were approved by the ethical committee of the University of Murcia (CEEA 288/2017).
HostingRepository	PRIDE
AnnounceDate	2021-05-24
AnnouncementXML	Submission_2021-05-24_02:17:52.204.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anita Horvatic
SpeciesList	scientific name: Equus caballus; NCBI TaxID: 9796;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-07 03:09:14	ID requested
⏵ 1	2021-05-24 02:17:52	announced
2	2023-11-14 08:52:06	announced	2023-11-14: Updated project metadata.

10.3390/ANI11051304;

submitter keyword: acute abdominal disease, horse, saliva, proteomics, TMT

Peter David Eckersall
contact affiliation	VetMedZg Laboratory,Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Institute of Biodiversity, Animal Health & Comparative Medicine, College of Medicine, Veterinary Medicine and Life Sciences, University of Glasgow, Glasgow, UK
contact email	David.Eckersall@glasgow.ac.uk
lab head
Anita Horvatic
contact affiliation	University of Zagreb, Faculty of Veterinary Medicine
contact email	horvatic.ani@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/05/PXD025241

PRIDE project URI

• PRIDE
  1. PXD025241
     1. Label: PRIDE project
     2. Name: Tandem mass tag-based proteomic analysis in saliva of horses with acute abdominal disease