PXD025226 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | An XPC-PARP axis links ALC1-mediated chromatin remodeling to global genome DNA damage repair |
Description | Cells employ global genome DNA damage repair (GGR) to eliminate a broad spectrum of DNA lesions, including those induced by UV light. The lesion-recognition factor XPC initiates repair of helix-distorting DNA lesions, but binds inefficiently to lesions that cause poor helix distortion. How such difficult-to-repair lesions are detected in chromatin is unknown. Here, we identify the poly-(ADP-ribose) polymerases PARP1 and PARP2 as constitutive interactors of XPC. The close interaction between these proteins results in the PARylation of XPC at UV lesions, and an XPC-dependent stimulation of the poly-(ADP-ribose) response, which facilitates the recruitment of the poly-(ADP-ribose)-dependent chromatin remodeler ALC1. Both ALC1 and in particular PARP2 are required for the efficient clearing of difficult-to-repair DNA lesions. Our study offers key insights into the molecular mechanisms of GGR by revealing a molecular bookmarking system, which primes chromatin containing difficult-to-repair DNA lesions for efficient repair. |
HostingRepository | PRIDE |
AnnounceDate | 2022-07-15 |
AnnouncementXML | Submission_2022-07-15_00:36:56.428.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Román González-Prieto |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-04-06 19:44:35 | ID requested | |
⏵ 1 | 2022-07-15 00:36:56 | announced | |
2 | 2023-11-14 08:52:18 | announced | 2023-11-14: Updated project metadata. |
Publication List
Keyword List
submitter keyword: PARP2, PARP1, XPC,GG-NER, ALC1 |
Contact List
Román González-Prieto |
contact affiliation | Cell and Chemical Biology Leiden University Medical Center The Netherlands |
contact email | R.Gonzalez_Prieto@lumc.nl |
lab head | |
Román González-Prieto |
contact affiliation | Leiden University Medical Center (LUMC) |
contact email | roman.gonzalez@cabimer.es |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD025226
- Label: PRIDE project
- Name: An XPC-PARP axis links ALC1-mediated chromatin remodeling to global genome DNA damage repair