PXD025219-2

PXD025219 is an original dataset announced via ProteomeXchange.

Title	Bacterial RF3 Senses Chaperone Function in Co-translational Folding
Description	Molecular chaperones assist in protein folding by interacting with nascent polypeptide chains (NCs) during translation, but whether the ribosome can sense chaperone defects and abort translation of misfolding NCs has not been explored. Here we used quantitative proteomics in E. coli to investigate the ribosome-associated chaperone network and the consequences of its dysfunction. Trigger factor and the DnaK (Hsp70) system are the major NC-binding chaperones. HtpG (Hsp90), GroEL and ClpB contribute increasingly, when DnaK is deficient. Surprisingly, misfolding of NCs due to defects in co-translational chaperone function or amino acid analog incorporation, results in recruitment of the non-canonical release factor RF3 to the ribosome. RF3 then cooperates with RF2 in mediating premature chain termination of a subset of abundant NCs, allowing their efficient degradation. This function of RF3 reduces the accumulation of misfolded proteins. It is critical for proteostasis maintenance and cell growth under conditions of limited chaperone availability.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:52:19.570.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Roman Körner
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-06 07:13:05	ID requested
1	2022-12-09 07:08:16	announced
⏵ 2	2023-11-14 08:52:20	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Molecular chaperones, co-translational folding

F. Ulrich Hartl
contact affiliation	Max-Planck Institute of Biochemistry, Department of Cellular Biochemistry
contact email	uhartl@biochem.mpg.de
lab head
Roman Körner
contact affiliation	Max-Planck Institute of Biochemistry, Department of Cellular Biochemistry
contact email	rkoerner@biochem.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD025219
     1. Label: PRIDE project
     2. Name: Bacterial RF3 Senses Chaperone Function in Co-translational Folding