PXD025180-1

PXD025180 is an original dataset announced via ProteomeXchange.

Title	White matter integrity requires continuous myelin synthesis at the inner tongue
Description	Myelin, the electrically insulating axonal sheath, is composed of lipids and proteins with exceptionally long lifetime. Using 3D electron microscopy, mass spectrometry imaging, and quantitative proteome analysis, we addressed the question how myelin function is affected by myelin turnover. We studied the integrity of myelinated tracts after experimentally preventing the formation of new myelin in the CNS of adult mice by an inducible myelin basic protein (Mbp) null allele. Recombined oligodendrocytes continued to express myelin genes, but failed to establish MBP-dependent compaction of myelin sheaths. Abundance changes of myelin proteins upon Mbp ablation were analyzed in whole optic nerve lysates at different time points and compared with shiverer mice. This naturally occurring mutant is inable to developmentally form compact myelin due to the lack of MBP and served as proxy for the demyelination endpoint. Label-free protein quantification using an ion mobility-enhanced data-independent acquisition (DIA) workflow with alternating low and elevated energy (referred to as UDMS^E) revealed that numerous myelin proteins are reduced in abundance, while microglia and astrocyte markers were increased, indicative of neuropathology. Taken together, we observed an axonal pathology with about 50% myelin loss after 20 weeks and concluded that functional axon-myelin units require the continuous incorporation of new myelin membranes.
HostingRepository	PRIDE
AnnounceDate	2022-01-14
AnnouncementXML	Submission_2022-01-14_05:40:57.762.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Olaf Jahn
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-06 03:16:02	ID requested
⏵ 1	2022-01-14 05:40:58	announced

Dataset with its publication pending

submitter keyword: optic nerve, myelin turnover, myelin basic protein (MBP), whole optic nerve proteome, juxtaparanode, shiverer, myelinoid body, FIB-SEM, 3D reconstruction, NanoSIMS

PD Dr. Olaf Jahn
contact affiliation	Proteomics Group Max-Planck-Institute of Experimental Medicine Hermann-Rein-Str. 3 37075 Goettingen Germany
contact email	jahn@em.mpg.de
lab head
Olaf Jahn
contact affiliation	Proteomics Group, Max-Planck-Institute of Experimental Medicine, Göttingen
contact email	jahn@em.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/01/PXD025180

PRIDE project URI

• PRIDE
  1. PXD025180
     1. Label: PRIDE project
     2. Name: White matter integrity requires continuous myelin synthesis at the inner tongue