PXD025158-1

PXD025158 is an original dataset announced via ProteomeXchange.

Title	Mapping microproteins and ncRNA-encoded polypeptides in different mouse tissues
Description	Small open reading frame encoded peptides (SEPs), also called microproteins, play a vital role in biological processes. Plenty of their open reading frames are located on the non-coding RNA (ncRNA) range. Recent research has demonstrated that ncRNA-encoded polypeptides have essential functions and exist ubiquitously in various tissues. To better understand the role of microproteins and ncRNA-encoded polypeptide expression in different tissues, we profiled the proteomic characterization of five mouse tissues by mass spectrometry, including bottom-up, top-down, and de novo sequencing strategies. Bottom-up and top-down with database-dependent searches identified 791 microproteins in the OpenProt database. De novo sequencing obtained 309 microproteins and ten ncRNA-encoded polypeptides that were not in the SEP database. In this study, we discovered 1100 microproteins in total, including 208 ncRNA-encoded polypeptides. From the annotation of these microproteins, we found that the brain contains the largest number of neuropeptides, while the spleen contains the most immunoassociated microproteins.
HostingRepository	iProX
AnnounceDate	2021-04-03
AnnouncementXML	Submission_2021-04-05_05:15:27.969.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ni Pan
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-05 05:14:47	ID requested
⏵ 1	2021-04-05 05:15:28	announced
2	2023-02-09 20:07:13	announced	2023-02-10: Update publication information.

Dataset with its publication pending

submitter keyword: Small open reading frame, microprotein, ncRNAs, tissue, polypeptides,

Cuihong Wan
contact affiliation	Central China Normal University
contact email	ch_wan@mail.ccnu.edu.cn
lab head
Ni Pan
contact affiliation	Central China Normal University
contact email	15071457583@163.com
dataset submitter

iProX dataset URI