PXD025087-1

PXD025087 is an original dataset announced via ProteomeXchange.

Title	Genomic and proteomic study of Andreprevotia ripae isolated from an anthill reveals an extensive repertoire of chitinolytic enzymes
Description	Chitin is an abundant natural polysaccharide that is hard to degrade because of its crystalline nature and because it is often found embedded in robust co-polymeric materials containing other polysaccharides, proteins and minerals. Thus, it is of interest to study the enzymatic machineries of highly specialized microbes found in chitin-rich environments. We describe a genomic and proteomic analysis of the chitinolytic machinery of Andreprevotia ripae, a Gram-negative bacterium that was isolated from an anthill. The genome of A. ripae encodes four secreted family GH19 chitinases of which two were detected, both being upregulated during growth on chitin. In addition, the genome encodes 25 secreted GH18 chitinases, of which 17 were detected and 12 were upregulated during growth on chitin. Finally, the single lytic polysaccharide monooxygenase (LPMO) of A. ripae, predicted to be chitin-active based on sequence characteristics and the presence of chitin-binding domains, was strongly upregulated during growth on chitin. Whereas 66 % of the 29 secreted chitinases contained two carbo¬hydrate-binding modules (CBMs) known for binding to chitin, this fraction was 93 % (13 out of 14) for the chitinases that were upregulated during growth on chitin, suggesting an important role for these CBMs. Next to demonstrating an unprecedentedly large multiplicity of upregulated chitinases, the present study also revealed several chitin-induced proteins that contain chitin-binding domains but lack a known catalytic function. These proteins are interesting targets for discovery of enzymes used by Nature to convert chitin-rich biomass.
HostingRepository	PRIDE
AnnounceDate	2021-06-10
AnnouncementXML	Submission_2021-06-10_01:27:10.904.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Magnus Arntzen
SpeciesList	scientific name: Andreprevotia sp. IGB-42; NCBI TaxID: 2497473;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-29 12:22:47	ID requested
⏵ 1	2021-06-10 01:27:11	announced

Dataset with its publication pending

submitter keyword: chitin, cazymes, Andreprevotia ripae

Vincent G. H. Eijsink
contact affiliation	Faculty of Chemistry, Biotechnology and Food Science, NMBU - Norwegian University of Life Sciences, N-1433 Ås, Norway
contact email	vincent.eijsink@nmbu.no
lab head
Magnus Arntzen
contact affiliation	Norwegian University of Life Sciences
contact email	magnus.arntzen@nmbu.no
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD025087
     1. Label: PRIDE project
     2. Name: Genomic and proteomic study of Andreprevotia ripae isolated from an anthill reveals an extensive repertoire of chitinolytic enzymes