PXD025081-2

PXD025081 is an original dataset announced via ProteomeXchange.

Title	Quantitative analysis of acetylation in Vero cells infected with Peste des petits ruminants virus
Description	Peste des petits ruminants virus (PPRV) is a negative-stranded RNA virus belonging to the Paramyxoviridae family and causes acute, highly contagious disease in small ruminants. Lysine acetylation plays central role in regulating gene expression. However, the extent and function of lysine acetylation in host cells during PPRV infection remains unknown. In this study, intensive proteomic quantification analysis of the proteome and acetylome of PPRV-infected Vero cells was performed using dimethylation labeling-based quantitative proteomics. As results, we identified 4729 proteins and 1068 acetylated proteins with 2641 quantified modification sites detected by mass spectrometry, of which 304 acetylated proteins with 410 acetylation sites were significantly acetylated in response to PPRV infection. Bioinformatics analyses revealed that the differentially acetylated proteins participated in carbohydrate catabolic and DNA metabolic process, and were associated with multifarious functions, suggesting that intracellular activities were extensively changed after PPRV infection. Protein-protein interaction network of the identified proteins further indicated that a variety of chaperone and ribosome processes were modulated by acetylation. To our knowledge, this is the first study on acetylome in host cell infected with PPRV. It provides an important point for future studies on the acetylated proteins involved in the host response to PPRV replication.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:07:22.164.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	xuelian meng
SpeciesList	scientific name: Chlorocebus sabaeus; NCBI TaxID: 60711;
ModificationList	acetylated residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-29 12:19:11	ID requested
1	2023-10-24 11:14:17	announced
⏵ 2	2023-11-14 09:07:23	announced	2023-11-14: Updated project metadata.
3	2024-10-22 06:08:54	announced	2024-10-22: Updated project metadata.

Meng X, Wang X, Zhu X, Zhang R, Zhang Z, Sun Y, Quantitative analysis of acetylation in peste des petits ruminants virus-infected Vero cells. Virol J, 20(1):227(2023) [pubmed]

submitter keyword: post-translational modification；lysine acetylation； protein-protein interaction；Peste des petits ruminants virus

Xuelian meng
contact affiliation	Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences
contact email	mengxuelian@caas.cn
lab head
xuelian meng
contact affiliation	Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences
contact email	mengxuelian@caas.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD025081
     1. Label: PRIDE project
     2. Name: Quantitative analysis of acetylation in Vero cells infected with Peste des petits ruminants virus