PXD025066-1

PXD025066 is an original dataset announced via ProteomeXchange.

Title	Furan warheads for covalent trapping of weak protein-protein interactions: specific cross-linking of thymosin β4 to actin
Description	We describe furan as a triggerable ‘warhead’ for site-specific cross-linking using the actin and thymosin β4 (Tβ4)-complex as model of a weak and dynamic protein-protein interaction with known 3D structure and with application potential in disease contexts. The presented in vitro validation of covalently acting ‘furan-armed’ Tβ4-variants provides initial proof to further exploit furan-technology for covalent drug design.
HostingRepository	PRIDE
AnnounceDate	2021-05-13
AnnouncementXML	Submission_2021-05-13_05:03:08.435.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD025066
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Marina Gay
SpeciesList	scientific name: Oryctolagus sp. 'Rabbit_OD'; NCBI TaxID: 2665958;
ModificationList	monohydroxylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-29 06:48:22	ID requested
⏵ 1	2021-05-13 05:03:08	announced

Dataset with its publication pending

submitter keyword: Furan, protein-protein interactions, covalent, cross-link, cross-linked peptide, actin, Thymosin β4, mass spectrometry

Annemieke Madder
contact affiliation	Organic and Biomimetic Chemistry Research Group, Department of Organic and Macromolecular Chemistry, Faculty of Science, Ghent University, Krijgslaan 281 S4, B-9000 Ghent, Belgium
contact email	Annemieke.Madder@Ugent.be
lab head
Marina Gay
contact affiliation	Institute for Research in Biomedicine
contact email	marina.gay@irbbarcelona.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/05/PXD025066

PRIDE project URI

• PRIDE
  1. PXD025066
     1. Label: PRIDE project
     2. Name: Furan warheads for covalent trapping of weak protein-protein interactions: specific cross-linking of thymosin β4 to actin