PXD025064 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A cryo-EM structure of metazoan TRAPPIII, the multisubunit complex that activates the GTPase Rab1 |
Description | The TRAPP complexes are nucleotide exchange factors that have essential roles in membrane traffic and autophagy. TRAPPII activates Rab11, and TRAPPIII activates Rab1, with the two complexes sharing a core of small subunits that affect nucleotide exchange, but being distinguished by specific large subunits that are essential for activity in vivo. Crystal structures of core subunits have revealed the mechanism of Rab activation, but how the core and the large subunits assemble to form the complexes was unknown. We report a cryo-EM structure of the entire TRAPPIII complex from Drosophila. The TRAPPIII-specific subunits TRAPPC8 and TRAPPC11 hold the catalytic core like a pair of tongs, with TRAPPC12 and TRAPPC13 positioned at the joint between them. TRAPPC2 and TRAPPC2L link the core to the two large arms, with the interfaces containing residues affected by disease-causing mutations. The TRAPPC8 arm is positioned such that it would contact Rab1 that is bound to the core, indicating how the arm could determine the specificity of the complex. A lower resolution structure of TRAPPII shows a similar architecture, and suggests that the TRAPP complexes evolved from a single ur-TRAPP. |
HostingRepository | PRIDE |
AnnounceDate | 2021-06-03 |
AnnouncementXML | Submission_2021-06-03_08:29:58.324.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Mark Skehel |
SpeciesList | scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF-X |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-03-29 04:42:17 | ID requested | |
⏵ 1 | 2021-06-03 08:29:59 | announced | |
Publication List
Galindo A, Planelles-Herrero VJ, Degliesposti G, Munro S, Cryo-EM structure of metazoan TRAPPIII, the multi-subunit complex that activates the GTPase Rab1. EMBO J, 40(12):e107608(2021) [pubmed] |
Keyword List
submitter keyword: Exchange factor / Rab1 / autophagy / membrane traffic / Golgi |
Contact List
Sean Munro |
contact affiliation | MRC Laboratory of Molecular Biology Francis Crick Avenue Cambridge CB2 0QH United Kingdom |
contact email | sean@mrc-lmb.cam.ac.uk |
lab head | |
Mark Skehel |
contact affiliation | MRC LMB |
contact email | mskehel@mrc-lmb.cam.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD025064
- Label: PRIDE project
- Name: A cryo-EM structure of metazoan TRAPPIII, the multisubunit complex that activates the GTPase Rab1