PXD025005-2

PXD025005 is an original dataset announced via ProteomeXchange.

Title	Quantitative Urinary Proteomics of UCPPS
Description	Urinary chronic pelvic pain syndrome (UCPPS) is a condition of unknown etiology characterized by pelvic pain, and urinary frequency and/or urgency. As the proximal fluid of this disease, urine is an ideal candidate sample matrix for an unbiased study of UCPPS. In this study, a large, discovery-phase, TMT-based quantitative urinary proteomics analysis of 244 subjects was performed. The subjects included patients with UCPPS (n=82), healthy controls (HC) (n=94) and other chronic pain diseases, termed positive controls (PC) (n=68). Utilizing training and testing cohorts , we identified and validated a small and distinct set of proteins that distinguished UCPPS from HC (n=7) and UCPPS from PC (n=3). Validated UCPPS:HC proteins were predominantly ECM/ECM modifying or immunomodulatory/host defense in nature. Significantly,varying proteins in the UCPPS:HC comparison were overrepresented by members of several dysregulated biological processes including decreased immune cell migration, decreased development of epithelial tissue and increased bleeding. Comparison with the PC cohort enabled evaluation of UCPPS-specific upstream regulators, contrasting UCPPS with other conditions that cause chronic pain. Specific to UCPPS were alterations in the predicted signaling of several upstream regulators, including alpha-catenin, IL6, EGF and TGFB1, among others. These findings advance our knowledge of the etiology of UCPPS and inform potential future clinical translation into a diagnostic panel for UCPPS.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:53:27.151.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	John Froehlich
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-25 08:08:48	ID requested
1	2021-11-29 06:18:19	announced
⏵ 2	2023-11-14 08:53:27	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Urine, prostatitis, interstitial cystitis

Richard S. Lee
contact affiliation	Boston Children's Hospital, Boston, MA. Department of Urology and Harvard Medical School, Boston, MA. Department of Surgery
contact email	Richard.Lee@childrens.harvard.edu
lab head
John Froehlich
contact affiliation	Children's Hospital Boston
contact email	john.froehlich@childrens.harvard.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD025005
     1. Label: PRIDE project
     2. Name: Quantitative Urinary Proteomics of UCPPS