PXD024983-1

PXD024983 is an original dataset announced via ProteomeXchange.

Title	Bifunctional dehalogenases in Dehalococcoides mediate complete debromination of penta- and tetra-brominated diphenyl ethers
Description	Polybrominated diphenyl ethers (PBDEs) are persistent, highly toxic, and widely distributed environmental pollutants. The microbial populations and functional reductive dehalogenases (RDases) responsible for PBDEs debromination in anoxic systems remain poorly understood, which confounds bioremediation of PBDE-contaminated sites. Here we report a PBDE-debrominating enrichment culture dominated by a previously undescribed Dehalococcoides mccartyi population. A D. mccartyi strain, designated TZ50, whose genome contains 25 putative RDase encoding genes was isolated from the debrominating enrichment culture. Strain TZ50 dehalogenated a mixture of penta- and tetra-BDE congeners (total BDEs 1.48 uM) to diphenyl ether within two weeks (0.58 uM Br- /d) via ortho- and meta- bromine elimination; strain TZ50 also dechlorinated tetrachloroethene (PCE) to vinyl chloride and ethene (260.2 M Cl- /d). Native-PAGE, proteomic profiling, and in vitro enzymatic activity assays implicated the involvement of three RDases in PBDEs and PCE dehalogenation. Two RDases, TZ50_0172 (PteATZ50) and TZ50_1083 (TceATZ50), were responsible for debromination of penta- and tetra-BDEs to di-BDE. TZ50_0172 and TZ50_1083 were also implicated in dechlorination of PCE to TCE and of TCE to vinyl chloride/ethene, respectively. The other expressed dehalogenase, TZ50_0090, was associated with debromination of di-BDE to diphenyl ether, but its role in PCE dechlorination was unclear. Comparatively few RDases are known to be involved in PBDE debromination and the identification of PteATZ50, TceATZ50, and TZ50_0090 provides additional information for evaluating debromination potential at contaminated sites. Moreover, the bifunctionality of the PteATZ50 and TceATZ50 in both PBDEs and PCE dehalogenation makes strain TZ50 a suitable candidate for remediation of co-contaminated sites.
HostingRepository	PRIDE
AnnounceDate	2021-06-16
AnnouncementXML	Submission_2021-06-15_18:03:16.335.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Siyan Zhao
SpeciesList	scientific name: Dehalococcoides mccartyi; NCBI TaxID: 61435;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-25 02:52:27	ID requested
⏵ 1	2021-06-15 18:03:16	announced

Dataset with its publication pending

submitter keyword: Brominated flame retardants, polybrominated diphenyl ethers, reductive debromination, Dehalococcoides, reductive dehalogenase (RDase)

Jianzhong He
contact affiliation	Department of Civil and Environmental Engineering, National University of Singapore
contact email	jianzhong.he@nus.edu.sg
lab head
Siyan Zhao
contact affiliation	National University of Singapore
contact email	siyan.zhao@outlook.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/06/PXD024983

PRIDE project URI

• PRIDE
  1. PXD024983
     1. Label: PRIDE project
     2. Name: Bifunctional dehalogenases in Dehalococcoides mediate complete debromination of penta- and tetra-brominated diphenyl ethers