PXD024929-2

PXD024929 is an original dataset announced via ProteomeXchange.

Title	SILAC APMS anlysis of TSSC4 interactome
Description	U5 snRNP is a complex particle essential for RNA splicing. U5 snRNPs undergo an intricate biogenesis that ensures that only a fully mature particle assembles into a splicing competent U4/U6•U5 tri-snRNP and enters the splicing reaction. During splicing, U5 snRNP is substantially rearranged and leaves as a U5/PRPF19 post-splicing particle, which requires re-generation before a next round of splicing. Here, we show that a previously uncharacterized protein TSSC4 is a new component of U5 snRNP that promotes tri-snRNP formation. We provide evidence that TSSC4 associates with U5 snRNP chaperones, U5 snRNP and the U5/PRPF19 particle. Specifically, TSSC4 interacts with U5-specific proteins PRPF8, EFTUD2 and SNRNP200. We also identified TSSC4 domains critical for the interaction with U5 snRNP and the PRPF19 complex, as well as for TSSC4 function in tri-snRNP assembly. TSSC4 emerges as a specific chaperone that acts in U5 snRNP de novo biogenesis as well as post-splicing recycling.
HostingRepository	PRIDE
AnnounceDate	2021-06-11
AnnouncementXML	Submission_2021-07-20_00:52:41.007.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Franck Vandermoere
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-22 09:20:20	ID requested
1	2021-06-10 21:57:59	announced
⏵ 2	2021-07-20 00:52:41	announced	2021-07-20: Updated publication reference for PubMed record(s): 34131137.

Klime, š, ov, á K, Voj, á, č, kov, á J, Radivojevi, ć N, Vandermoere F, Bertrand E, Verheggen C, Stan, ě, k D, TSSC4 is a component of U5 snRNP that promotes tri-snRNP formation. Nat Commun, 12(1):3646(2021) [pubmed]

submitter keyword: APMS

SILAC

immunoprecipitation

David Stanek
contact affiliation	Institute of Molecular Genetics of the Czech Academy of Sciences
contact email	david.stanek@img.cas.cz
lab head
Franck Vandermoere
contact affiliation	IGF (Montpellier, France), CNRS, INSERM, University of Montpellier
contact email	franck.vandermoere@igf.cnrs.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/06/PXD024929

PRIDE project URI

• PRIDE
  1. PXD024929
     1. Label: PRIDE project
     2. Name: SILAC APMS anlysis of TSSC4 interactome