PXD024925-1

PXD024925 is an original dataset announced via ProteomeXchange.

Title	Discovery of metal-binding proteins by thermal proteome profiling.
Description	Metal-binding proteins (MBPs) play diverse and critical functions in all living systems and many human diseases are closely related to dysfunctional MBPs. Here we developed a chemoproteomic method named METAL-TPP for global discovery of MBPs in proteomes, which operates by extracting metals from MBPs with chelators and logging the resulting structural perturbation of MBPs with thermal proteome profiling. We applied METAL-TPP to the human proteomes and identified 2856 proteins with significant thermal shifts, including not only many known MBPs but also 75 proteins without previous annotation of metal-binding activity.
HostingRepository	iProX
AnnounceDate	2021-03-22
AnnouncementXML	Submission_2021-03-22_05:15:11.283.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xin Zeng
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-22 05:14:48	ID requested
⏵ 1	2021-03-22 05:15:11	announced

Dataset with its publication pending

submitter keyword: metal-binding proteins, thermal proteome profiling

Chu Wang
contact affiliation	Peking University
contact email	chuwang@pku.edu.cn
lab head
Xin Zeng
contact affiliation	Peking University
contact email	xinzeng@pku.edu.cn
dataset submitter

iProX dataset URI