PXD024909-1

PXD024909 is an original dataset announced via ProteomeXchange.

Title	OpenCell: Proteome-scale endogenous tagging enables the cartography of human cellular organization
Description	Mapping the global proteome circuitry of the human cell is one of the central goals of the post-genomic era. Here, we combine high-throughput genome engineering of ~1,300 cell lines endogenously tagged with fluorescent protein fusions, 3D live-cell imaging, mass spectrometry (MS)-based high-speed interactomics and advanced machine learning to decode the interaction and localization architecture of the human proteome. We delineate interacting protein families and facilitate unbiased biological discovery by unsupervised clustering, while hierarchical analyses of the interactome superimposed to localization uncover principles that template cellular organization. Furthermore, we discover that localization patterns alone are often enough to predict molecular interactions. ‘OpenCell’ is a global proteome-scale resource for human protein localization and interaction at endogenous expression levels. Our analytical methods are open-source and our data set is presented as an advanced interactive website (‘OpenCell’.czbiohub.org) to empower the community with the quantitative cartography of human cellular organization at proteome level.
HostingRepository	PRIDE
AnnounceDate	2022-03-15
AnnouncementXML	Submission_2022-03-14_20:35:03.524.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mario Oroshi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-22 04:36:30	ID requested
⏵ 1	2022-03-14 20:35:03	announced

Dataset with its publication pending

submitter keyword: High-throughput proteomics

High-speed proteomics

Interaction proteomics

Human interactome

Global human interactome map

Matthias Mann
contact affiliation	Department of Proteomics and Signal Transduction Max Planck Institute of Biochemistry Germany
contact email	mmann@biochem.mpg.de
lab head
Mario Oroshi
contact affiliation	Proteomics
contact email	oroshi@biochem.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/03/PXD024909

PRIDE project URI

• PRIDE
  1. PXD024909
     1. Label: PRIDE project
     2. Name: OpenCell: Proteome-scale endogenous tagging enables the cartography of human cellular organization