PXD024851-2

PXD024851 is an original dataset announced via ProteomeXchange.

Title	Display of the Human Mucinome with Defined O-Glycans by Gene Engineered Cells
Description	Mucins are a large family of heavily O-glycosylated proteins that cover all mucosal surfaces and constitute the major macromolecules in most body fluids. Mucins can form aggregated networks and bundles that serve as a barrier, but also assist in containing, feeding, clearing and replenishing microorganisms. Mucins are primarily defined by their variable tandem repeat (TR) domains that are densely decorated with different O-glycan structures in distinct patterns, and these arguably convey much of the informational content of mucins. However, the O-glycodomains have long evaded detailed structural and functional exploration. Here, we developed a cell-based platform for the display and production of human TR O-glycodomains (~200 amino acids) with tunable structures and patterns of O-glycans using membrane-bound and secreted constructs expressed in glycoengineered HEK293 cells. The expressed mucin TRs revealed surprisingly high fidelity in complete decoration with designed O-glycan structures, which enabled intact mass analysis with the simplest glycoforms. Availability of defined mucin TR O-glycodomains advances experimental studies into the versatile role of mucins at the interface with pathogenic microorganisms and the microbiome, and sparks new strategies for molecular dissection of specific roles of adhesins, glycoside hydrolases, glycopeptidases, viruses and other interactions with mucin TRs as highlighted by examples.
HostingRepository	PRIDE
AnnounceDate	2021-05-11
AnnouncementXML	Submission_2021-07-19_22:45:44.893.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sergey Vakhrushev
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Hex-HexNAc
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-19 00:00:21	ID requested
1	2021-05-11 03:39:51	announced
⏵ 2	2021-07-19 22:45:45	announced	2021-07-20: Updated publication reference for PubMed record(s): 34210959.

Nason R, B, ü, ll C, Konstantinidi A, Sun L, Ye Z, Halim A, Du W, S, ø, rensen DM, Durbesson F, Furukawa S, Mandel U, Joshi HJ, Dworkin LA, Hansen L, David L, Iverson TM, Bensing BA, Sullam PM, Varki A, Vries E, de Haan CAM, Vincentelli R, Henrissat B, Vakhrushev SY, Clausen H, Narimatsu Y, Display of the human mucinome with defined O-glycans by gene engineered cells. Nat Commun, 12(1):4070(2021) [pubmed]

submitter keyword: Intact Mass, OrbiTrap, Mucin, Glycosylation

Sergey Vakhrushev
contact affiliation	Copenhagen Center for Glycomics (CCG), Glyco-MS Group Department of Cellular and Molecular Medicine University of Copenhagen
contact email	seva@sund.ku.dk
lab head
Sergey Vakhrushev
contact affiliation	Department of Cellular and Molecular Medicine
contact email	seva@sund.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/05/PXD024851

PRIDE project URI

• PRIDE
  1. PXD024851
     1. Label: PRIDE project
     2. Name: Display of the Human Mucinome with Defined O-Glycans by Gene Engineered Cells