PXD024813-1

PXD024813 is an original dataset announced via ProteomeXchange.

Title	Nasal septum deviation as the consequence of BMP-controlled changes to cartilage properties
Description	Nasal septum deviation (NSD) is a common abnormality of the septal cartilage often associated with disordered breathing. The etiology of NSD is unknown. BMP7 neural crest-knockout mice, a well-characterized model for midfacial hypoplasia and nasal airway obstruction, develops a deviated septum by 4 weeks of age. Using comparative gene expression, quantitative proteomics, and immunofluorescence analysis we investigated the septum prior, immediately preceding, and with established deviation. We provide a detailed description of cellular and molecular changes leading to septum deviation, including changes to extracellular matrix organization, cell metabolism, and chondrocyte differentiation. Loss of BMP7 was associated with acquisition of elastic cartilage properties, a switch to glucose metabolism, and molecular characteristics commonly associated with osteoarthritis (OA). Many alterations preceded the deviation establishing that molecular changes to chondrocyte properties predispose to the deviation. Interestingly, NSD was associated with a persistent increase in BMP2. Genetic reduction of BMP2 in BMP7ncko mice restores WNT signalling, energy metabolism, and rescues NSD, showing the importance of balanced BMP signaling for normal cartilage. Many of the cellular and molecular changes have been described for knee osteoarthritis, suggesting that these two pathologies might have a similar underlying etiology.
HostingRepository	PRIDE
AnnounceDate	2021-04-30
AnnouncementXML	Submission_2021-04-30_10:42:35.858.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Antoine Dufour
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; deamidated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-18 00:41:38	ID requested
⏵ 1	2021-04-30 10:42:36	announced

Dataset with its publication pending

submitter keyword: cartilage, septum, BMP

Antoine Dufour
contact affiliation	University of Calgary, Department of Physiology and Pharmacology
contact email	antoine.dufour@ucalgary.ca
lab head
Antoine Dufour
contact affiliation	University of Calgary Department of Physiology and Pharmacology
contact email	antoine.dufour@ucalgary.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD024813
     1. Label: PRIDE project
     2. Name: Nasal septum deviation as the consequence of BMP-controlled changes to cartilage properties