PXD024766-1

PXD024766 is an original dataset announced via ProteomeXchange.

Title	Site-specific Identification and Quantification of Protein Glutathionylation under Ischemic Stress
Description	Ischemia or ischemic reperfusion is well-known for its contribution to heart diseases. During ischemia and reperfusion, a flux of nutrients and oxygen to cardiomyocytes is altered, which causes a burst of ROS from mitochondria and other enzymes. Elevated levels of ROS can cause oxidative modifications of cardiac proteins, such as protein glutathionylation. Despite previous extensive studies, proteomic identification of glutathionylated proteins in cardiomyocytes under altered levels of nutrients and oxygen has been relatively limited. We have applied our clickable glutathione approach to HL-1 cardiomyocyte cell line under metabolic alterations of glucose, oxygen, and fatty acids to detect and identify proteins undergoing glutathionylation.
HostingRepository	PRIDE
AnnounceDate	2021-08-13
AnnouncementXML	Submission_2021-08-13_00:40:37.124.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maheeshi Yapa Abeywardana
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-16 04:57:58	ID requested
⏵ 1	2021-08-13 00:40:38	announced

Yapa Abeywardana M, Samarasinghe KTG, Munkanatta Godage D, Ahn YH, Identification and Quantification of Glutathionylated Cysteines under Ischemic Stress. J Proteome Res, 20(9):4529-4542(2021) [pubmed]

submitter keyword: Gutathionylation, Cardiomyocytes, Click chemistry

Young-Hoon Ahn
contact affiliation	Wayne State University Chemistry Department, Ahn Lab, United States of America (Lab head)
contact email	yahn@chem.wayne.edu
lab head
Maheeshi Yapa Abeywardana
contact affiliation	wayne state university
contact email	fr6217@wayne.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/08/PXD024766

PRIDE project URI

• PRIDE
  1. PXD024766
     1. Label: PRIDE project
     2. Name: Site-specific Identification and Quantification of Protein Glutathionylation under Ischemic Stress