PXD024751-1
PXD024751 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | PROTEOMIC ANALYSIS OF THE UMBILICAL CORD IN FETAL GROWTH RESTRICTION AND PREECLAMPSIA |
| Description | Fetal growth restriction (FGR) is associated with adverse perinatal outcomes. Pre-eclampsia (PreE) increases the associated perinatal morbidity and mortality. The structure of the umbilical cord in the setting of FGR and PreE has yet to be determined. This study aimed to examine changes in the umbilical cord (UC) composition in pregnancies complicated by FGR and FGR with PreE. UC from gestational age-matched pregnancies with isolated FGR (n = 5), FGR with PreE (n = 5) and controls (n = 5) were collected, and a portion of the UC was processed for histologic and proteomic analysis. Manual segmentation analysis was performed to measure cross-section areas of umbilical cord regions. Wharton's Jelly samples were analyzed on a tims-TOF Pro. Spectral count and ion abundance data were analyzed simultaneously, creating an intersection dataset from a combination of multiple mass spectrometry search and inference engines. UCs from FGR and FGR with PreE had lower cross-sectional area and Wharton's Jelly area compared with control (p = ;0.03). When comparing isolated FGR to control, 28 proteins were significantly different in abundance analysis and 34 in spectral count analysis (p-value < 0.05). Differential expression analysis between PreE with FGR vs controls, demonstrated 48 proteins were significantly different in abundance and 5 in spectral count (p-value < 0.05). The majority of proteomic changes occur in proteins associated with extracellular matrix, cellular process, inflammatory, and angiogenesis pathways. |
| HostingRepository | MassIVE |
| AnnounceDate | 2022-03-03 |
| AnnouncementXML | Submission_2022-03-03_06:45:00.129.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Dr. Miranda Gardner |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | L-methionine sulfoxide; S-carboxamidomethyl-L-cysteine |
| Instrument | timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2021-03-15 10:36:15 | ID requested | |
| ⏵ 1 | 2022-03-03 06:45:01 | announced |
Publication List
| Conrad MS, Gardner ML, Miguel C, Freitas MA, Rood KM, Ma'ayeh M, Proteomic analysis of the umbilical cord in fetal growth restriction and preeclampsia. PLoS One, 17(2):e0262041(2022) [pubmed] |
Keyword List
| submitter keyword: Wharton's Jelly, Umbilical Cord, Pre-eclampsia, Fetal Growth Restriction (FGR), Bottom-Up Proteomics, LC-MS/MS, timsTOF Pro |
Contact List
| Kara M. Rood | |
|---|---|
| contact affiliation | The Ohio State University |
| contact email | kara.rood@osumc.edu |
| lab head | |
| Dr. Miranda Gardner | |
| contact affiliation | The Ohio State University |
| contact email | gardner.207@osu.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000087051/ |
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