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PXD024748-1

PXD024748 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePolyomic analyses of dopaminergic neurons isolated from human substantia nigra in Parkinson’s disease: An exploratory study.
DescriptionDopaminergic neurons of the substantia nigra pars compacta selectively and progressively degenerate in Parkinson’s disease. Until now, molecular analyses of dopaminergic neurons in PD have been limited to genomic and transcriptomic approaches, whereas, to the best of our knowledge, no proteomic or combined polyomic study examining the protein profile of these neurons, is currently available. In this exploratory study, we used laser microdissection to extract dopaminergic neurons from 10 human SNpc samples obtained at autopsy in Parkinson’s disease patients and control subjects. Extracted RNA and proteins were identified by RNA sequencing and nano-LC-MS/MS, respectively, and the differential expression between Parkinson’s disease and control group was assessed. Qualitative analyses confirmed that the microdissection protocol preserves the integrity of our samples and offers access to specific molecular pathways. This polyomic analysis highlighted differential expression of 52 genes and 33 proteins, including molecules of interest already known to be dysregulated in Parkinson’s disease, such as LRP2, PNMT, CXCR4, MAOA and CBLN1 genes, or the Aldehyde dehydrogenase 1 protein. On the other hand, despite the same samples were used for both analyses, correlation between RNA and protein expression was low, as exemplified by the CST3 gene encoding for the cystatin C protein. This is the first exploratory study analyzing both gene and protein expression of LMD-dissected neurons from substantia nigra pars compacta in Parkinson’s disease.
HostingRepositoryPRIDE
AnnounceDate2021-12-30
AnnouncementXMLSubmission_2021-12-30_14:35:16.042.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAffif Zaccaria
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-03-15 08:57:27ID requested
12021-12-30 14:35:16announced
Publication List
Zaccaria A, Antinori P, Licker V, K, ö, vari E, Lobrinus JA, Burkhard PR, Multiomic Analyses of Dopaminergic Neurons Isolated from Human Substantia Nigra in Parkinson's Disease: A Descriptive and Exploratory Study. Cell Mol Neurobiol, 42(8):2805-2818(2022) [pubmed]
Keyword List
submitter keyword: Parkinson’s disease, human brain tissue, dopaminergic neurons, laser micro-dissection, Transcriptomics, Proteomics .
Contact List
Pierre R. Burkhard
contact affiliationNeuroproteomics Group, University Medical Center, Faculty of Medicine, Geneva University, Geneva, Switzerland
contact emailpierre.burkhard@hcuge.ch
lab head
Affif Zaccaria
contact affiliationUniversity of Geneva
contact emailaffif.zaccaria@unige.ch
dataset submitter
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Dataset FTP location
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PRIDE project URI
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