PXD024731-2
PXD024731 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Transcriptional signature in microglia associated with Aβ plaque phagocytosis |
Description | The role of microglia cells in Alzheimer’s disease (AD) is well recognized, however their molecular and functional diversity remain unclear. Here we isolated amyloid plaque-containing (using labelling with methoxy–XO4, XO4+) and non-containing (XO4-) microglia from an AD mouse model. Transcriptomics analysis identified different transcriptional trajectories in ageing and AD mice. XO4+ microglial transcriptomes demonstrated dysregulated expression of genes associated with late onset AD. We further showed that the transcriptional program associated with XO4+ microglia from mice is present in a subset of human microglia isolated from brains of individuals with AD. XO4- microglia displayed transcriptional signatures associated with accelerated ageing and contained more intracellular post-synaptic material than XO4+ microglia, despite reduced active synaptosome phagocytosis. We identified HIF1α as potentially regulating synaptosome phagocytosis in vitro using primary human microglia, and BV2 mouse microglial cells. Together these findings provide insight into molecular mechanisms underpinning the functional diversity of microglia in AD. |
HostingRepository | PRIDE |
AnnounceDate | 2021-04-12 |
AnnouncementXML | Submission_2021-05-28_06:12:12.839.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Nathan Croft |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | iodoacetamide derivatized residue |
Instrument | TripleTOF 6600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2021-03-15 05:22:25 | ID requested | |
1 | 2021-04-12 08:07:53 | announced | |
⏵ 2 | 2021-05-28 06:12:13 | announced | 2021-05-28: Updated publication reference for PubMed record(s): 34021136. |
Publication List
Grubman A, Choo XY, Chew G, Ouyang JF, Sun G, Croft NP, Rossello FJ, Simmons R, Buckberry S, Landin DV, Pflueger J, Vandekolk TH, Abay Z, Zhou Y, Liu X, Chen J, Larcombe M, Haynes JM, McLean C, Williams S, Chai SY, Wilson T, Lister R, Pouton CW, Purcell AW, Rackham OJL, Petretto E, Polo JM, plaque phagocytosis. Nat Commun, 12(1):3015(2021) [pubmed] |
Keyword List
submitter keyword: Alzheizer's disease, microglia, TripleToF 6600 |
Contact List
Nathan Croft | |
---|---|
contact affiliation | Biomedicine Discovery Institute and Department of Biochemistry & Molecular Biology, Monash University, Melbourne, VIC, Australia |
contact email | nathan.croft@monash.edu |
lab head | |
Nathan Croft | |
contact affiliation | Monash University |
contact email | nathan.croft@monash.edu |
dataset submitter |
Full Dataset Link List
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/04/PXD024731 |
PRIDE project URI |
Repository Record List
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