PXD024688-2

PXD024688 is an original dataset announced via ProteomeXchange.

Title	IL-22 Prevents Epithelial Dysfunction During Citrobacter rodentium-induced Infectious Colitis
Description	IBD is a complex autoimmune disease characterized by dysregulated interactions between host immune responses and microbiome at the intestinal epithelium interface. Here we identified shared protein alterations in intestinal epithelial differentiation and function between IBD and Citrobacter rodentium infected FVB mice. We discovered that prophylactic treatment with the mucosal healing therapy IL-22.Fc in the infected FVB mice reduced disease severity and rescued the mice from lethality. Notably, we observed an emergence of intermediate undifferentiated intestinal epithelial cells upon infection, with disrupted expression of the solute transporter machinery as well as components critical for intestinal barrier integrity. Multi-omics analyses revealed that with IL-22.Fc treatment several disease associated changes were prevented (including disruption of the solute transporter machinery), and proper physiological homeostatic functions of the intestine was restored. Taken together, we unveiled the disease relevance of the C. rodentium induced colitis model to IBD and demonstrated the protective role of the mucosal healing therapy IL-22.Fc in ameliorating the epithelial dysfunction.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:59:32.541.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD024688
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Liang Jin
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-11 23:10:08	ID requested
1	2023-10-05 15:02:44	announced
⏵ 2	2023-11-14 07:59:32	announced	2023-11-14: Updated project metadata.
3	2024-10-22 06:05:00	announced	2024-10-22: Updated project metadata.

10.6019/PXD024688;

submitter keyword: Citrobacter,Mouse, HFX

Yu Tian
contact affiliation	Drug Metablism and Pharmackinetics, Abbvie Bioresearch Center
contact email	yu.tian@abbvie.com
lab head
Liang Jin
contact affiliation	AbbVie Bioresearch Center
contact email	liangjin0912@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD024688

PRIDE project URI

• PRIDE
  1. PXD024688
     1. Label: PRIDE project
     2. Name: IL-22 Prevents Epithelial Dysfunction During Citrobacter rodentium-induced Infectious Colitis