PXD024669-1

PXD024669 is an original dataset announced via ProteomeXchange.

Title	MALDI-MS Profiling of Intact Blood Proteins without the Need for Freezing - Dried Serum Spots as Future Clinical Tools for Patient Screening
Description	To open new ways for MALDI-MS-based patient screening, blood serum is the most preferred specimen because of its richness in patho-physiological information and due to ease of collection. To overcome deleterious freeze / thaw cycles and to reduce high costs for shipping and storage, we sought to develop a procedure which enables MALDI-MS protein profiling of blood serum proteins without the need for serum freezing. MALDI mass spectra from either (p)FFS or (p)DSS preparations showed on average 25 strong ion signals (mass range m/z 6000 to m/z 10,000) from intact serum proteins (apolipoproteins, complement proteins, transthyretin and hemoglobin) and from proteolytic processing products. Semi-quantitative analysis of three ion pairs: m/z 6433 and 6631, m/z 8205 and 8916, as well as m/z 9275 and 9422 indicated that the mass spectra from either (p)FFS or (p)DSS contained the same protein composition information. A workflow that avoids the conventional cold-chain and yet enables to investigate intact serum proteins and/or serum proteolysis products by MALDI-MS profiling was developed. The here described protocol tremendously broadens clinical application of MALDI-MS and simultaneously allows to reduce the costs for storage and shipping of serum samples.
HostingRepository	PRIDE
AnnounceDate	2021-05-20
AnnouncementXML	Submission_2021-05-20_01:34:14.339.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Kreutzer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Bruker Daltonics flex series

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-11 02:20:29	ID requested
⏵ 1	2021-05-20 01:34:14	announced
2	2021-05-20 04:11:04	announced	2021-05-20: Updated publication reference for PubMed record(s): 33955049.

Dataset with its publication pending

submitter keyword: MALDI-MS profiling, Fast Frozen Serum, Dried Serum Spots, intact serum protein analysis, serum proteolysis analysis

Michael O. Glocker
contact affiliation	Proteome Center Rostock, Medical Faculty and Natural Science Faculty, University of Rostock, Schillingallee 69, 18059 Rostock, Germany
contact email	michael.glocker@med.uni-rostock.de
lab head
Michael Kreutzer
contact affiliation	University Medical Center Rostock
contact email	michael.kreutzer@med.uni-rostock.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/05/PXD024669

PRIDE project URI

• PRIDE
  1. PXD024669
     1. Label: PRIDE project
     2. Name: MALDI-MS Profiling of Intact Blood Proteins without the Need for Freezing - Dried Serum Spots as Future Clinical Tools for Patient Screening