PXD024614-1

PXD024614 is an original dataset announced via ProteomeXchange.

Title	Comparative Phosphoproteomics Reveals a Role for AMPK in Hypoxia Signaling in Testes of Oriental River Prawn (Macrobrachium nipponense)
Description	Hypoxia is one of the major stresses in aquaculture animals. Recently, we reported that hypoxia disrupts the endocrine system and inhibits testicular function of oriental river prawns (Macrobrachium nipponense), but the molecular mechanism of testes responded to hypoxia remains largely unknown. In the present study, we aimed to integrate whole phosphoproteomic profiles of hypoxia-treated testes of the oriental river prawn (Macrobrachium nipponense). We successfully isolated sperm cells, and evaluated mitochondrial morphology and function using laser confocal microscopy, flow cytometry, and biochemical analyses. Quantitative proteomics identified 117 differentially abundant phosphorylated proteins, and these proteins are mainly involved in the pathways related to cellular processes, including autophagy, apoptosis, FoxO signaling pathway. Protein–protein interaction analysis clustered these phosphoproteins into three groups, many of which have been suggested to impact carbohydrate metabolism, autophagy and signal regulation in testes. Western blotting confirmed that phosphorylated proteins including AMPK, ULK1, and TP53 (of the AMPK pathway) may contribute to testicular dysfunction caused by hypoxia. Further, we investigated the potential roles of AMP-activated protein kinase (AMPK)’s in testes mitochondrial autophagy and apoptosis in M. nipponense as induced by hypoxia. Simultaneous knockdown of AMPKα in sperm cells led to a decrease in FOXO3a phosphorylation at Ser413, upregulation of caspase-3 and caspase-9 activities, and an increased apoptosis rate. These results improve our understanding of hypoxia-induced energy metabolism disorders in the testes of M. nipponense.
HostingRepository	iProX
AnnounceDate	2021-03-09
AnnouncementXML	Submission_2021-03-29_21:57:43.432.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shengming Sun
SpeciesList	scientific name: Macrobrachium nipponense; NCBI TaxID: 159736;
ModificationList	iTRAQ8plex reporter+balance reagent acylated residue; phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-09 20:33:08	ID requested
⏵ 1	2021-03-29 21:57:43	announced
2	2023-08-28 00:27:41	announced	2023-08-28: Update information.

Dataset with its publication pending

submitter keyword: Phosphoproteomics

Shengming Sun
contact affiliation	Key Laboratory of Freshwater Aquatic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University
contact email	sunshengming621416@163.com
lab head
Shengming Sun
contact affiliation	Key Laboratory of Freshwater Aquatic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University
contact email	sunshengming621416@163.com
dataset submitter

iProX dataset URI