PXD024601-1

PXD024601 is an original dataset announced via ProteomeXchange.

Title	Systematic analysis of the impact of R-methylation on RBPs-RNA interactions
Description	RNA binding proteins (RBPs) bind RNAs through specific RNA-binding domains, generating multi-molecular complexes known as ribonucleoproteins (RNPs). Various post-translational modifications (PTMs) have been described to regulate RBP structure, subcellular localization and interactions with other proteins or RNAs. Recent proteome-wide experiments showed that RBPs are the most representative group within the class of arginine (R)-methylated proteins; moreover, emerging evidence suggests that this modification plays a major role in the regulation of RBP-RNA interaction. Nevertheless, a systematic analysis of how changes in protein-R-methylation can affect globally RBPs-RNA interactions has not yet been carried out. We describe here a quantitative proteomics approach to profile global changes of RBP-RNA interactions upon the modulation of protein arginine methyltransferases PRMT1 and PRMT5. By coupling the recently described Orthogonal Organic Phase Separation (OOPS) with SILAC labelling and pharmacological modulation of PRMT1 and PRMT5 enzyme, we describe the RNA-binding protein dynamics in dependence of protein-R-methylation.
HostingRepository	PRIDE
AnnounceDate	2021-08-25
AnnouncementXML	Submission_2021-08-25_06:13:04.347.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Enrico Massignani
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monomethylated residue; methylated residue; dimethylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-09 01:28:52	ID requested
⏵ 1	2021-08-25 06:13:04	announced

Dataset with its publication pending

submitter keyword: OOPS, R-methylation, RBPs, PRMTs

Tiziana Bonaldi
contact affiliation	European Institute of Oncology IRCSS, Department of Experimental Oncology, Milan, Italy
contact email	tiziana.bonaldi@ieo.it
lab head
Enrico Massignani
contact affiliation	European Institute of Oncology (IEO)
contact email	enrico.massignani@ieo.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/08/PXD024601

PRIDE project URI

• PRIDE
  1. PXD024601
     1. Label: PRIDE project
     2. Name: Systematic analysis of the impact of R-methylation on RBPs-RNA interactions