PXD024590-1

PXD024590 is an original dataset announced via ProteomeXchange.

Title	Analysis of the substrate specificities and reaction kinetics of the yeast oligosaccharyltransferase isoforms
Description	Oligosaccharyltransferase (OST) catalyzes the central step in N-linked protein glycosylation, the transfer of a pre-assembled oligosaccharide from its lipid carrier onto asparagine residues of secretory proteins. The prototypic hetero-octameric OST complex from the yeast Saccharomyces cerevisiae exists as two isoforms that contain either Ost3p or Ost6p. These two OST complexes have different protein substrate specificities in vivo. The two OST complexes were purified from genetically engineered strains expressing only one isoform. The kinetic properties and substrate specificities were characterized using a quantitative in vitro glycosylation assay with short peptides and different synthetic lipid-linked oligosaccharide (LLO) substrates. We showed that the peptide sequence close to the glycosylation sequon affected peptide affinity and turnover rate. The length of the lipid moiety affected LLO affinity, while the lipid double bond stereochemistry had a greater influence on LLO turnover rates. The two OST complexes had similar affinities for both the peptide and LLO substrates but showed significantly different turnover rates.
HostingRepository	PRIDE
AnnounceDate	2021-05-19
AnnouncementXML	Submission_2021-05-19_00:44:03.381.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD024590
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Chia-wei Lin
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	N-acetylhexosaminylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-08 22:54:27	ID requested
⏵ 1	2021-05-19 00:44:03	announced

Dataset with its publication pending

submitter keyword: yeast oligosaccharyltransferase, site-occupancy, substract specificity

Prof. Markus Aebi
contact affiliation	Institute of Microbiology, ETH Zurich
contact email	markus.aebi@micro.biol.ethz.ch
lab head
Chia-wei Lin
contact affiliation	Functional genomics center Zurich
contact email	lin.chiawei@fgcz.ethz.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD024590
     1. Label: PRIDE project
     2. Name: Analysis of the substrate specificities and reaction kinetics of the yeast oligosaccharyltransferase isoforms