<<< Full experiment listing

PXD024588-1

PXD024588 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProtein identification of spermatozoa and seminal plasma in bottlenose dolphin (Tursiops truncatus)
DescriptionProteins play an important role in many reproductive functions such as sperm maturation and physiology, sperm transit in the female genital tract or sperm-oocyte interaction. However, little information concerning reproductive features is available in the case of aquatic animals. The present study aims to characterize the proteome of both spermatozoa and seminal plasma of bottlenose dolphins (Tursiops truncatus) as a model organism for cetaceans. Ejaculate samples were obtained voluntarily from two trained dolphins housed in an aquarium. Spermatozoa and seminal plasma were analyzed by means of proteomic analyses using a LC-MS/MS and a list with the gene symbols corresponding to each protein was submitted to DAVID database. A total of 423 and 307 proteins were identified in spermatozoa and seminal plasma, respectively, and the samples shared 111 proteins. Furthermore, 70 proteins were identified as involved in reproductive processes, 39 in spermatozoa, and 31 in seminal plasma. The top-5 most abundant proteins were also identified in these samples: AKAP3, ODF2, TUBB, GST3, ROPN1 for spermatozoa and CST11, LTF, ALB, HSP90B1, PIGR for seminal plasma. In conclusion, this proteomic study provides the first characterization of proteomic expression patterns in cetacean sperm and seminal plasma, opening a window to future research on the discovery of biomarkers, analysis of conservation capacity or additional applications in the field of assisted reproduction technologies.
HostingRepositoryPRIDE
AnnounceDate2021-06-14
AnnouncementXMLSubmission_2021-06-14_01:00:44.735.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLuz Valero
SpeciesList scientific name: Tursiops truncatus truncatus; NCBI TaxID: 2663531;
ModificationListNo PTMs are included in the dataset
InstrumentTripleTOF 6600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-03-08 22:52:10ID requested
12021-06-14 01:00:45announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Cetacean
Proteomic
Semen
Sperm cells
Seminal plasma
Contact List
Mª José Izquierdo-Rico
contact affiliationDepartment of Cell Biology and Histology, Faculty of Medicine, Campus Mare Nostrum,University of Murcia, 30100 Murcia, IMIB, Spain
contact emailmjoseir@um.es
lab head
Luz Valero
contact affiliationUniversitat de Valencia
contact emailmluz.valero@uv.es
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/06/PXD024588
PRIDE project URI
Repository Record List
[ + ]