PXD024588 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Protein identification of spermatozoa and seminal plasma in bottlenose dolphin (Tursiops truncatus) |
Description | Proteins play an important role in many reproductive functions such as sperm maturation and physiology, sperm transit in the female genital tract or sperm-oocyte interaction. However, little information concerning reproductive features is available in the case of aquatic animals. The present study aims to characterize the proteome of both spermatozoa and seminal plasma of bottlenose dolphins (Tursiops truncatus) as a model organism for cetaceans. Ejaculate samples were obtained voluntarily from two trained dolphins housed in an aquarium. Spermatozoa and seminal plasma were analyzed by means of proteomic analyses using a LC-MS/MS and a list with the gene symbols corresponding to each protein was submitted to DAVID database. A total of 423 and 307 proteins were identified in spermatozoa and seminal plasma, respectively, and the samples shared 111 proteins. Furthermore, 70 proteins were identified as involved in reproductive processes, 39 in spermatozoa, and 31 in seminal plasma. The top-5 most abundant proteins were also identified in these samples: AKAP3, ODF2, TUBB, GST3, ROPN1 for spermatozoa and CST11, LTF, ALB, HSP90B1, PIGR for seminal plasma. In conclusion, this proteomic study provides the first characterization of proteomic expression patterns in cetacean sperm and seminal plasma, opening a window to future research on the discovery of biomarkers, analysis of conservation capacity or additional applications in the field of assisted reproduction technologies. |
HostingRepository | PRIDE |
AnnounceDate | 2021-06-14 |
AnnouncementXML | Submission_2021-06-14_01:00:44.735.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Luz Valero |
SpeciesList | scientific name: Tursiops truncatus truncatus; NCBI TaxID: 2663531; |
ModificationList | No PTMs are included in the dataset |
Instrument | TripleTOF 6600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-03-08 22:52:10 | ID requested | |
⏵ 1 | 2021-06-14 01:00:45 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Cetacean |
Proteomic |
Semen |
Sperm cells |
Seminal plasma |
Contact List
Mª José Izquierdo-Rico |
contact affiliation | Department of Cell Biology and Histology, Faculty of Medicine, Campus Mare Nostrum,University of Murcia, 30100 Murcia, IMIB, Spain |
contact email | mjoseir@um.es |
lab head | |
Luz Valero |
contact affiliation | Universitat de Valencia |
contact email | mluz.valero@uv.es |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD024588
- Label: PRIDE project
- Name: Protein identification of spermatozoa and seminal plasma in bottlenose dolphin (Tursiops truncatus)