PXD024559-1
PXD024559 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Identification of cellular proteins interacting with PEDV M protein through APEX2 labeling |
| Description | Membrane (M) proteins of coronaviruses are the most abundant component of the virus envelope and play crucial roles in virus assembly, virus budding and the regulation of host immunity. To understand more about these functions in the context of PEDV M protein, forty host cell proteins interacting with the M protein were identified in the present study by exploiting the proximity-labeling enzyme APEX2 (a mutant soybean ascorbate peroxidase). Bioinformatic analysis showed that the identified host cell proteins were related to fifty-four signal pathways and a wide diversity of biological processes. Interaction between M and five of the identified proteins (RIG-I, PPID, NHE-RF1, S100A11, CLDN4) was confirmed by co-immunoprecipitation (Co-IP). In addition, knockdown of PPID and S100A11 genes by siRNA significantly improved virus production, indicating that the proteins encoded by the two genes were interfering with or down-regulating virus replication in infected cells. Identification of the host cell proteins accomplished in this study provides new information about the mechanisms underlying PEDV replication and immune evasion. |
| HostingRepository | iProX |
| AnnounceDate | 2021-03-07 |
| AnnouncementXML | Submission_2021-03-06_22:01:42.231.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Shijuan Dong |
| SpeciesList | scientific name: Sus scrofa scrofa; NCBI TaxID: 415978; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2021-03-06 22:01:07 | ID requested | |
| ⏵ 1 | 2021-03-06 22:01:42 | announced | |
| 2 | 2023-02-19 23:37:36 | announced | 2023-02-20: Update publication information. |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: PEDV, membrane protein, cellular proteins, APEX2, interaction |
Contact List
| Zhen Li | |
|---|---|
| contact affiliation | Shanghai Academy of Agricultural Sciences |
| contact email | zhenli60@163.com |
| lab head | |
| Shijuan Dong | |
| contact affiliation | Institute of Animal Science and Veterinary Science, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai Academy of Agricultural Sciences (SAAS), Shanghai, PR China |
| contact email | dsjnm@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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