PXD024546 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic analysis of polysomes isolated from human cells infected with polio, zika or dengue viruses |
Description | Viral pathogens are an ongoing threat to public health worldwide. Dissecting their dependence on host biosynthetic pathways could lead to effective antiviral therapies. To define how entero- and flaviviruses redirect host ribosomes to synthesize viral proteins and disable host protein production, we performed proteomic analysis of lysates and isolated polysomes from human Huh7 cells infected with either polio, zika or dengue viruses. We find that infection remodels polysome composition along similar principles, without major changes to core ribosome stoichiometry. These viruses use different strategies to evictfrom polysomes a common set of translation initiation and RNA surveillance factors while recruiting host machineries specifically required for viral biogenesis. We also find that both zika and dengue utilize the collagen prolyl-hydroxylation machinery to mediate co-translational modification of conserved prolines in the viral polyprotein. Our findings show how RNA viruses co-opt polysome modularity and establish a powerful strategy to identify targets for selective antiviral interventions. |
HostingRepository | PRIDE |
AnnounceDate | 2021-08-17 |
AnnouncementXML | Submission_2021-09-15_23:19:01.472.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ranen Aviner |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-03-05 03:37:58 | ID requested | |
1 | 2021-08-17 02:22:36 | announced | |
⏵ 2 | 2021-09-15 23:19:02 | announced | 2021-09-16: Updated publication reference for PubMed record(s): 34408324. |
Publication List
Aviner R, Li KH, Frydman J, Andino R, Cotranslational prolyl hydroxylation is essential for flavivirus biogenesis. Nature, 596(7873):558-564(2021) [pubmed] |
Keyword List
submitter keyword: LC-MS/MS, ribosomes, polysomes, human, viruses, infection, translation, protein synthesis, protein folding, proteostasis |
Contact List
Raul Andino |
contact affiliation | Department of Microbiology and Immunology University of California, San Francisco (UCSF) USA |
contact email | raul.andino@ucsf.edu |
lab head | |
Ranen Aviner |
contact affiliation | Stanford University |
contact email | ranen.aviner@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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- PRIDE
- PXD024546
- Label: PRIDE project
- Name: Proteomic analysis of polysomes isolated from human cells infected with polio, zika or dengue viruses